Abstract: Disclosed are a carrier, a vacuumizing device, and a tissue cryopreservation system. The carrier is provided with at least one freezing chamber for loading a tissue, and a contact surface of the freezing chamber that comes into contact with the tissue is provided with a plurality of flow guide holes. The carrier may cooperate with the vacuumizing device so as to suck away excess liquid, such as a protective agent, on a surface of the tissue by vacuum, and discharge the liquid from the freezing chamber through the flow guide holes, thereby reducing the toxicity damage that may be caused by prolonged contact between the tissue and a high-concentration protective agent.