Abstract: Provided is a reaction cup, comprising cup walls and a cup bottom. The cup walls are provided with clamping portions, the clamping portions can allow a membrane strip to stand upright in the reaction cup, and the connecting area of the two opposite clamping portions forms an upright placement position for the membrane strip. The present invention further provides a molecular hybridization instrument used with the reaction cup, and an analyte testing method. The reaction cup can allow the membrane strip to stand upright therein, and can be opened for operation, thereby reducing the area occupied by the reaction cup on the molecular hybridization instrument, and reducing the area of the molecular hybridization instrument. A plurality of reaction cups can be placed on the molecular hybridization instrument at the same time, which increases the number of tests and improves the utilization and working efficiency of the molecular hybridization instrument.