Abstract: Provided is a microfluidic device comprising an incubation layer, the incubation layer including at least one dock, each of the at least one dock defines a stepped tank comprising an upper tank and a lower tank, an inflow channel in fluid communication with the stepped tank for supplying a fluid to the stepped tank, and an outflow channel in fluid communication with the stepped tank for draining the fluid from the stepped tank, wherein the geometry of the upper tank is configured to allow culturing of a fish larva therein, and wherein the geometry of the lower tank is configured to reversibly receive the fish larva from the upper tank and to dock the fish larva at a controlled orientation for imaging or observation.

Abstract: Provided is a microfluidic device comprising an incubation layer, the incubation layer including at least one dock, each of the at least one dock defines a stepped tank comprising an upper tank and a lower tank, an inflow channel in fluid communication with the stepped tank for supplying a fluid to the stepped tank, and an outflow channel in fluid communication with the stepped tank for draining the fluid from the stepped tank, wherein the geometry of the upper tank is configured to allow culturing of a fish larva therein, and wherein the geometry of the lower tank is configured to reversibly receive the fish larva from the upper tank and to dock the fish larva at a controlled orientation for imaging or observation.

Abstract: A microfluidic system for separating an analyte from a sample fluid including a series of fluidic channels including at least one first region and at least one second region. The first region includes a plurality of L-nodes, which connects to each other in series. The second region includes a plurality of R-nodes, which connects to each other in series. The first region is configured to trigger at least about one lamination process cycle for both the sample fluid and the buffer fluid and the second region is configured to trigger at least about one reverse lamination process cycle for both the sample fluid and the buffer fluid, whereby the lamination process cycle and the reverse lamination process cycle causes the analyte to diffuse to the buffer fluid from the sample fluid. A method for separating the analyte is also disclosed.

Abstract: A microfluidic system for separating an analyte from a sample fluid including a series of fluidic channels including at least one first region and at least one second region. The first region includes a plurality of L-nodes, which connects to each other in series. The second region includes a plurality of R-nodes, which connects to each other in series. The first region is configured to trigger at least about one lamination process cycle for both the sample fluid and the buffer fluid and the second region is configured to trigger at least about one reverse lamination process cycle for both the sample fluid and the buffer fluid, whereby the lamination process cycle and the reverse lamination process cycle causes the analyte to diffuse to the buffer fluid from the sample fluid. A method for separating the analyte is also disclosed.