Abstract: The present disclosure provides a rapid three-dimensional imaging system based on a multi-angle 4Pi microscope. The system includes: an illumination module, configured to obtain a parallel light of which a size covering a projection surface of a spatial light modulator; a wavefront modulation module, configured to place the LCOS device on a Fourier plane of an illumination end; a two-dimensional scanning module, configured to control a light beam to realize a two-dimensional scanning on an object plane; an illumination interference module, configured to generate point spread function PSFs of a 4Pi through an illumination interference to irradiate a fluorescent sample; an imaging module, configured to acquire interference images of two fluorescent signals; and a controller, configured to control the wavefront modulation module to adjust a polarization direction of the light to generate PSFs of the 4Pi with different inclination angles.

Abstract: A microscopic imaging system and a microscopic imaging method. The system includes: an illumination module configured to generate a laser illumination, an LCOS device located in a Fourier plane of the laser illumination and configured to modulate a phase of the laser illumination, a 4-F system configured to adjust a size of a light beam of the laser illumination, an excitation lens group configured to generate a point illumination focused in a sample plane, a detecting lens group configured to capture an image of a PSF of the point illumination, a camera sensor, and a controller configured to synchronously control a change in a phase pattern of the LCOS device and an image capture of the camera sensor.

Abstract: The present disclosure provides a rapid three-dimensional imaging system based on a multi-angle 4Pi microscope. The system includes: an illumination module, configured to obtain a parallel light of which a size covering a projection surface of a spatial light modulator; a wavefront modulation module, configured to place the LCOS device on a Fourier plane of an illumination end; a two-dimensional scanning module, configured to control a light beam to realize a two-dimensional scanning on an object plane; an illumination interference module, configured to generate point spread function PSFs of a 4Pi through an illumination interference to irradiate a fluorescent sample; an imaging module, configured to acquire interference images of two fluorescent signals; and a controller, configured to control the wavefront modulation module to adjust a polarization direction of the light to generate PSFs of the 4Pi with different inclination angles.

Abstract: Provided are a microscopic imaging system and a microscopic imaging method. The system includes: an illumination module configured to generate a laser illumination, an LCOS device located in a Fourier plane of the laser illumination and configured to modulate a phase of the laser illumination, a 4-F system configured to adjust a size of a light beam of the laser illumination, an excitation lens group configured to generate a point illumination focused in a sample plane, a detecting lens group configured to capture an image of a PSF of the point illumination, a camera sensor, and a controller configured to synchronously control a change in a phase pattern of the LCOS device and an image capture of the camera sensor.