Abstract: In a method for isolating analytes of a first analyte class, which are extracellular vesicles and/or circulating tumor cells, and analytes of a second analyte class, which are cell-free nucleic acids, from the same sample volume of a biological sample in a centrifugal microfluidic system, the sample volume is guided into a first isolation chamber of a fluidics module containing a first isolation structure so that the analytes of the first analyte class are retained by the first isolation structure while the analytes of the second analyte class are not retained and pass through the first isolation structure as part of a residual liquid, which is passed into a second isolation chamber of the fluidics module containing a second isolation structure so that the analytes of the second analyte class are retained by the second isolation structure. The analytes of the first analyte class and the second analyte class are separated from the respective isolation structure to provide the analytes for subsequent analysis.

Abstract: A fluidics module has first fluidics structures having a first fluid chamber, second fluidics structures having a second fluid chamber, a first connection having a fluidic resistance between the first fluid chamber and the second fluid chamber, third fluidics structures having a third fluid chamber, a second connection having a barrier between the first fluid chamber and the third fluid chamber, a pressure compensation channel between the second fluid chamber and the third fluid chamber, and fourth fluidics structures having a fourth fluid chamber connected to the second fluidics structures via a third connection. Liquid can be transferred centrifugally from the first fluid chamber into the second fluid chamber. Liquid from the second fluid chamber can be transferred under rotation into the fourth fluid chamber via the third connection to generate negative pressure in the third fluid chamber.