Patents by Inventor Gustav José Rodrigo
Gustav José Rodrigo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240018184Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: ApplicationFiled: July 18, 2023Publication date: January 18, 2024Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20230333098Abstract: A separation system, and an elution arrangement to be provided in a separation system, for separating a biomolecule from a cell culture are provided.Type: ApplicationFiled: May 15, 2023Publication date: October 19, 2023Inventors: Ola Lind, Johan Fredrik Avallin, Andreas Torbjorn Lundin, Nils Norrman, Ronnie Palmgren, Gustav Jose Rodrigo
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Patent number: 11753438Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: GrantFiled: May 10, 2017Date of Patent: September 12, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20230279047Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: April 11, 2023Publication date: September 7, 2023Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20230257417Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support: b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing said separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA).Type: ApplicationFiled: March 10, 2023Publication date: August 17, 2023Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
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Patent number: 11708390Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.Type: GrantFiled: May 10, 2017Date of Patent: July 25, 2023Assignee: Cytiva BioProcess R&D ABInventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 11686726Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.Type: GrantFiled: December 27, 2017Date of Patent: June 27, 2023Assignee: Cytiva Sweden ABInventors: Ola Lind, Johan Fredrik Avallin, Andreas Torbjorn Lundin, Nils Norrman, Ronnie Palmgren, Gustav Jose Rodrigo
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Patent number: 11685764Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: April 2, 2021Date of Patent: June 27, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 11623941Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucType: GrantFiled: May 27, 2020Date of Patent: April 11, 2023Assignee: CYTIVA BIOPROCESS R&D ABInventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
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Publication number: 20210221841Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: April 2, 2021Publication date: July 22, 2021Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 10995113Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: June 5, 2020Date of Patent: May 4, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20210087227Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO: 51 or SEQ ID NO: 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.Type: ApplicationFiled: December 8, 2020Publication date: March 25, 2021Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander
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Patent number: 10889615Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO: 4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.Type: GrantFiled: May 10, 2017Date of Patent: January 12, 2021Assignee: Cytiva BioProcess R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander
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Publication number: 20200299325Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.Type: ApplicationFiled: May 10, 2017Publication date: September 24, 2020Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20200299326Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: ApplicationFiled: June 5, 2020Publication date: September 24, 2020Inventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Publication number: 20200283475Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucType: ApplicationFiled: May 27, 2020Publication date: September 10, 2020Inventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
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Publication number: 20200239517Abstract: The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days.Type: ApplicationFiled: May 10, 2017Publication date: July 30, 2020Inventors: Annika Forss, Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 10711035Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: November 13, 2019Date of Patent: July 14, 2020Assignee: GE HEALTHCARE BIOPROCESS R&D ABInventors: Gustav José Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson
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Patent number: 10703774Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting ofType: GrantFiled: November 10, 2016Date of Patent: July 7, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Annika Kristina Forss, Gustav Jose Rodrigo, Tomas Bjorkman, Jesper Ulf Hansson, Mats Ander
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Patent number: 10654887Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.Type: GrantFiled: September 30, 2016Date of Patent: May 19, 2020Assignee: GE Healthcare Bio-Process R&D ABInventors: Gustav Jose Rodrigo, Tomas Bjorkman, Mats Ander, Jesper Ulf Hansson