Abstract: Disclosed is a process for producing a composition involving: (a) distilling fish oil in a vacuum distillation column to obtain a first residue and first distillate, and (b) distilling the first distillate in a vacuum distillation column to obtain a second distillate and a second residue that includes a composition containing cholesterol, eicosapentaenoic acid, and docosahexaenoic acid. Also disclosed is a process for producing a composition involving: (a) distilling fish oil in a vacuum distillation column to obtain a first residue and first distillate, (b) distilling the first distillate in a vacuum distillation column to obtain a second distillate and second residue containing cholesterol, (c) esterifying the second residue to obtain a mixture containing cholesterol esters, and (d) distilling the mixture in a vacuum distillation column to obtain a third distillate and a third residue that includes a composition containing cholesterol esters of eicosapentaenoic acid and docosahexaenoic acid.

Abstract: Disclosed is a process for producing a cholesterol concentrate including the steps of (a) distilling a fish oil having no more than 2% free fatty acids in an admixture with an auxiliary fluid in a vacuum distillation column to obtain a first distillate and a first residue; and (b) distilling the first distillate in a vacuum distillation column to obtain a second distillate and a second residue, wherein the second residue includes the cholesterol concentrate.