Abstract: Among the various aspects of the present disclosure is the provision of an electrophoresis and electroporation device, system, and methods of use thereof. Devices to administer drugs with electrophoresis and electroporation are described. Systems to administer drugs with an electrophoresis and electroporation device by applying voltages with a power source and controller are also described. Also disclosed are methods of administering a therapeutic agent with an electrophoresis and electroporation device or system.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: Devices and methods for joining a first and second tissue in a patient are disclosed that include a base with a plurality of recurved tines oriented to a tine axis and extending from a first surface of the base. The tines provide unidirectional traction of the first tissue along the tine axis toward the first surface. The first tissue is secured to the first surface of the device at the plurality of recurved tines and the second tissue is secured to the device at a second surface opposite the first surface to join the first and second tissues.

Abstract: Among the various aspects of the present disclosure is the provision of devices, systems, and methods for tracking the positions and orientations of at least one bone screw implanted within a surgical site. The disclosed device includes an extension that includes an extension base configured to couple to the bone screw, a marker arm coupled at one end to the extension base in a hinged arrangement, and an optical marker attached to a free end of the marker arm opposite the hinged attachment. The optical marker includes a pair of lenticular arrays in a coplanar arrangement in which the major axes of the lenticular arrays are mutually perpendicular. Each lenticular array is configured to display different hues at different viewing angles. The disclosed method includes transforming a single image of a surgical site containing at least one bone screw and extension into the global positions of each bone screw based on the pixel positions and hues corresponding to the lenticular arrays of each optical marker.

Abstract: Disclosed herein are methods of making a mold for use with cells, such as engineered tissue. The method includes printing a 3D printed resin mold, casting a hydrogel over the 3D printed resin mold to create a crosslinked hydrogel negative mold, and casting a silicone rubber elastomer over the hydrogel negative mold to create a master mold.

Abstract: Devices and methods for joining a first and second tissue in a patient are disclosed that include a base with opposed first and second surfaces, and a plurality of recurved tines oriented to a tine axis and extending from the first surface of the base. The base defines four suture holes extending through the first and second surfaces of the base and configured to receive at least one suture passing between the first and second surfaces of the base. The plurality of recurved tines provides unidirectional traction of the first tissue along the tine axis toward the first surface to secure the device to the first tissue. The device is secured to the second tissue at the second surface with the at least one suture secured to at least one anchor secured to the second tissue.

Abstract: The disclosure provides for a variable stiffness catheter. The catheter may include an inner layer forming an inner lumen, a middle layer surrounding the inner layer, and an outer layer surrounding the middle layer. The middle layer may include an annular lumen and a plurality of strings within the annular lumen, and the plurality of strings may be connected to the inner layer. Applying a vacuum and evacuating the annular lumen of the middle layer alters a frictional force between the inner layer and the outer layer and thereby changes the catheter's flexural rigidity.

Abstract: The disclosure provides for an isovolumetric pump system for the removal of a thrombus and a method of use thereof. The isovolumetric pump system includes at least one inflow container connected to an output of the treatment region, at least one outflow container connected to an input of the treatment region, and a drawbar connecting the inflow container and the outflow container. As the drawbar is drawn back, the same quantity of fluid is drawn from the treatment region into the inflow container and injected into the treatment region from the outflow container.

Abstract: The disclosure provides for an adjustable catheter system with isovolumetric suction and restoration of fluid for the removal of a thrombus and a method of use thereof. The catheter system includes an inner catheter and an outer sheath surrounding at least a portion of the inner catheter. The inner catheter may include at least three lumina extending from the proximal end to the distal end of the inner catheter, at least one infusion fenestration along the infusion segment, and a distal encapsulation balloon at the distal end. The outer sheath may include at least three lumina extending from the proximal end to the distal end of the outer sheath and a proximal encapsulation balloon at the distal end. The catheter system may further include an agitator for mechanical morcellation of the thrombus.

Abstract: An arteriovenous graft and methods of reducing the risk of graft thrombosis and extending patency of the arteriovenous graft are provided herein. The arteriovenous graft is operable for attaching to a vein at a venous anastomosis. In some aspects, the arteriovenous graft includes a plurality of grooves at a venous anastomosis end of the arteriovenous graft and the venous anastomoses may be arranged such that the arteriovenous graft and the vein meet at an angle of 30° or less.

Abstract: The disclosure provides for an adjustable catheter system with isovolumetric suction and restoration of fluid for the removal of a thrombus and a method of use thereof. The catheter system includes an inner catheter and an outer sheath surrounding at least a portion of the inner catheter. The inner catheter may include at least three lumina extending from the proximal end to the distal end of the inner catheter, at least one infusion fenestration along the infusion segment, and a distal encapsulation balloon at the distal end. The outer sheath may include at least three lumina extending from the proximal end to the distal end of the outer sheath and a proximal encapsulation balloon at the distal end. The catheter system may further include an agitator for mechanical morcellation of the thrombus.

Abstract: The disclosure provides for an adjustable catheter system with isovolumetric suction and restoration of fluid for the removal of a thrombus and a method of use thereof. The catheter system includes an inner catheter and an outer sheath surrounding at least a portion of the inner catheter. The inner catheter may include at least three lumina extending from the proximal end to the distal end of the inner catheter, at least one infusion fenestration along the infusion segment, and a distal encapsulation balloon at the distal end. The outer sheath may include at least three lumina extending from the proximal end to the distal end of the outer sheath and a proximal encapsulation balloon at the distal end. The catheter system may further include an agitator for mechanical morcellation of the thrombus.

Abstract: A stent for placement in a blood vessel with a wall having an aneurysm including an endovascular graft having a first end, a second end, and a tubular body that is expandable and extends from the first end to the second end. The stent also includes a coagulation apparatus attached to the tubular body between the first and second ends. The coagulation apparatus has a frame and a coagulant attached to the frame. When the stent is deployed within the blood vessel, the tubular body extends across the aneurysm and expands at the first and second ends to seal against the wall of the blood vessel such that blood is channeled across the aneurysm through the tubular body such that a pocket of blood is defined external to the tubular body. The frame expands into the pocket to orient the coagulant therein and promote coagulation of blood within the pocket.

Abstract: An arteriovenous graft and methods of reducing the risk of graft thrombosis and extending patency of the arteriovenous graft are provided herein. The arteriovenous graft is operable for attaching to a vein at a venous anastomosis. In some aspects, the arteriovenous graft includes a plurality of grooves at a venous anastomosis end of the arteriovenous graft and the venous anastomoses may be arranged such that the arteriovenous graft and the vein meet at an angle of 30° or less.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: A method of using a catheter assembly for inserting in a fluid filled space in a body includes providing a main body having a first end portion and a second end portion. The first end portion is positioned within the fluid filled space. The second end portion is adjusted to extend outwardly from the fluid filled space when the first end portion is positioned within the fluid filled space. A catheter tip is connected to the second end portion of the main body. The catheter tip includes a housing having a cavity defined therein and a rotating element positioned within the fluid filled space. The rotating element is rotated within the cavity of the housing to impart movement of the first end portion of the main body within the fluid filled space.

Abstract: A self-cleaning endoscope and methods of using the same are described. The self-cleaning endoscope includes a housing having a distal end and a proximal end opposite the distal end, a lens positioned at the distal end of the housing, and a lens protector coupled to the distal end of the housing external to the lens. The self-cleaning endoscope also includes a shaft extending longitudinally through the housing. The shaft is operatively coupled to the lens protector and configured to rotate the lens protector about an axis of rotation coaxial with the shaft. The self-cleaning endoscope further includes a motor at the proximal end of the housing. The motor is operatively coupled to the shaft and configured to drive rotation of the shaft.

Abstract: Provided herein are compositions and methods for treating a subject with damaged tissue, such as an injury associated with a tissue to tissue (e.g., a connective tissue-to-connective tissue or tissue to bone) interface. One aspect provides an adhesive film or adhesive layer, optionally comprising a biomaterial, tissue growth factors, including CTGF/CCN2, or cells.

Abstract: A breakaway connector generally includes a first valve having a first rotating-type fluid control member, and a second valve that is attachable to the first valve and has a second rotating-type fluid control member. Each of the fluid control members has an open position in which fluid is permitted to flow through the respective valve, and a closed position in which fluid is prevented from flowing through the respective valve. Each one of the valves is configured to engage the fluid control member of the other one of the valves to move the fluid control member from the open position to the closed position upon detachment of the valves from one another.

Abstract: A stent for placement in a blood vessel with a wall having an aneurysm including an endovascular graft having a first end, a second end, and a tubular body that is expandable and extends from the first end to the second end. The stent also includes a coagulation apparatus attached to the tubular body between the first and second ends. The coagulation apparatus has a frame and a coagulant attached to the frame. When the stent is deployed within the blood vessel, the tubular body extends across the aneurysm and expands at the first and second ends to seal against the wall of the blood vessel such that blood is channeled across the aneurysm through the tubular body such that a pocket of blood is defined external to the tubular body. The frame expands into the pocket to orient the coagulant therein and promote coagulation of blood within the pocket.