Abstract: The present invention relates to a DNA probe adapted to be used as a PCR target for the detection of the presence of C. stellatoidea and a subgroup of C. albicans strains in a biological sample, which comprises the nucleic acid sequence set forth in SEQ ID NO:1 or any functional analogs thereof wherein the hybridization of the probe and the rRNA of C. albicans is substantially preserved. The present invention also relates to a method for the detection of trace amount of C. stellatoidea or a subgroup of C. albicans in a biological sample, which comprises the steps of: a) isolating DNA from said biological sample; b) amplifying said isolated DNA of step a) with paired oligonucleotides: 5' primer: 5' AAC TTA GAA CTG GTA CGG 3' (SEQ ID NO:2), 3' primer: 5' AGT AGA TAG GGA CAG TGG 3' (SEQ ID No:3); or 5' GAC TCT CAA CCT ATA AGG 3' (SEQ ID NO:4), 3' primer: 5' TTA AGC ATT GCT CCA AGA 3' (SEQ ID NO:5); c) isolating amplified DNA of step b) and determining the presence or absence of C.