Abstract: Disclosed is an improved process for the production of fatty acid lower alkyl esters according to formula (I) R1CO—OR2??(I) in which R1CO represents a linear or branched acyl moiety having from about 6 to about 22 carbon atoms and 0 to 6 double bonds and wherein R2 is a linear or branched alkyl moiety having 1 to 6 carbon atoms, by transesterification of triglycerides or fatty acid esters using C1-C6 aliphatic alcohols, which includes the steps of (a) providing an aqueous mixture of aliphatic C1-C6 alcohol by fermenting in a first reactor a carbon source comprising carbohydrates and/or glycerol produce a fermentation broth comprising said aliphatic C1-C6 alcohol, (b) providing in a second reactor a triglyceride and/or an ester of a fatty acid, together with a biocatalyst capable of effecting a transesterification reaction, (c) transferring said aqueous aliphatic C1-C6 alcohol mixture obtained from said first reactor into said second reactor to provide a two-phase system, and (d) effecting the transesteri

Abstract: The invention concerns compositions and methods combining at least two phytases for hydrolyzing phytic acid (myo-inositol hexakis phosphate) into inorganic monophosphates, into myo-inositols with lower degree of phosphorylation and into free myo-inositol. Said compositions and methods are of particular interest for animal feeding.

Abstract: A reaction medium for enzyme-catalyzed reactions is provided, comprising an oil-in-water emulsion including water, an emulsifier, an oil phase and at least one interfacially active enzyme, where the emulsion is produced by the phase inversion temperature process and has a droplet size of 50 to 400 nm. A method for the enzyme-catalyzed esterification, transesterification or hydrolysis of fatty acid alkyl esters and/or triglycerides is also provided where the oil-in-water emulsion is used as the reaction medium.

Abstract: Polypeptides comprising a polypeptide chosen from the group consisting of the polypeptide of SEQ ID No. 2 and a polypeptide exhibiting at least 90% identity with the polypeptide of SEQ ID No. 2. Polynucleotides selected from the group consisting of the polynucleotide whose sequence is between position 1538 and position 2923 of SEQ ID No. 1, and a polynucleotide encoding the polypeptide chosen from the group consisting of the polypeptide of SEQ ID No. 2 and a polypeptide exhibiting at least 90% identity with the polypeptide of SEQ ID No. 2. Furthermore, vectors and expression cassettes comprising the polynucleotide.

Abstract: A reaction medium for enzyme-catalyzed reactions is provided, comprising an oil-in-water emulsion including water, an emulsifier, an oil phase and at least one interfacially active enzyme, where the emulsion is produced by the phase inversion temperature process and has a droplet size of 50 to 400 nm. A method for the enzyme-catalyzed esterification, transesterification or hydrolysis of fatty acid alkyl esters and/or triglycerides is also provided where the oil-in-water emulsion is used as the reaction medium.

Abstract: The invention concerns compositions and methods combining at least two phytases for hydrolyzing phytic acid (myo-inositol hexakis phosphate) into inorganic monophosphates, into myo-inositols with lower degree of phosphorylation and into free myo-inositol. Said compositions and methods are of particular interest for animal feeding.

Abstract: The invention concerns a gene coding for a Debaryomyces castellii phytase which hydrolyzes the six phosphate bonds of phytate. The invention also concerns the uses of said phytase in nutritional additives or foods for animals for improving their digestibility and nutritional value.

Abstract: Disclosed is an improved process for the production of fatty acid lower alkyl esters according to formula (I) R1CO—OR2 ??(I) in which R1CO represents a linear or branched acyl moiety having from about 6 to about_22 carbon atoms and 0 to 6 double bonds and wherein R2 is a linear or branched alkyl moiety having 1 to 6 carbon atoms, by transesterification of triglycerides or fatty acid esters using C1-C6 aliphatic alcohols, which includes the steps of (a) providing an aqueous mixture of aliphatic C1-C6 alcohol by fermenting in a first reactor a carbon_source comprising carbohydrates and/or glycerol produce a fermentation broth comprising said aliphatic C1-C6 alcohol, (b) providing in a second reactor a triglyceride and/or an ester of a fatty acid, together with a biocatalyst capable of effecting a transesterification reaction, (c) transferring said aqueous aliphatic C1-C6 alcohol mixture obtained from said first reactor into said_second reactor to provide_a two-phase system, and (d) effecting the transeste

Abstract: An oil in water emulsion including water, an emulsifier and an oil phase is produced by the phase inversion temperature process and has a droplet size of 50 to 400 nm is provided. A process for the enzyme-catalyzed esterification, transesterification or hydrolysis of fatty acid alkyl esters and/or triglycerides is also provided where the oil in water emulsion is used as the reaction medium.

Abstract: The invention relates to polypeptides having perhydrolase activity with an amino acid sequence which is at least 80% homologous or at least 65% identical to the amino acid sequence shown in SEQ ID No. 3, with the exception of SEQ ID NO. 3. The invention also relates to polypeptides having perhydrolase activity which contain at least one motif which is at least 50% homologous or at least 70% identical to a motif selected from the group consisting of SEQ ID NO. 4: GYSGGxxAxxWAxxxxxxYAPE, SEQ ID NO 5: GYSGGxxAxxWAxxxxxxYAPD, SEQ ID NO 6: GFSGGxxAxxWAxxxxxxYAPE, SEQ ID NO 7: GFSGGxxAxxWAxxxxxxYAPD, SEQ ID NO 8: GYSGGxxAxxWAxxxxxxYA and SEQ ID NO 9: GFSGGxxAxxWAxxxxxxYA.

Abstract: Polypeptides with lipase/acyltransferase activity are described with an amino acid sequence which has identity to the sequence reported in SEQ ID NO 2 of at least 49%, also polypeptides which display this activity, as well as nucleic acids (genes) which code for these polypeptides, vectors which contain nucleic acids which code for these polypeptides, transformed microorganisms which contain these nucleic acids, processes for production of these polypeptides and the application of nucleic acids for discovering new lipase/acyltransferases and the use of these lipase/acyltransferases as catalysts in chemical and biochemical processes.

Abstract: Polypeptides with lipase/acyltransferase activity are described with an amino acid sequence which has identity to the sequence reported in SEQ ID NO 2 of at least 49%, also polypeptides which display this activity, as well as nucleic acids (genes) which code for these polypeptides, vectors which contain nucleic acids which code for these polypeptides, transformed microorganisms which contain these nucleic acids, processes for production of these polypeptides and the application of nucleic acids for discovering new lipase/acyltransferases and the use of these lipase/acyltransferases as catalysts in chemical and biochemical processes.

Abstract: The invention concerns a product for adjusting the degree of alcohol of wines or alcoholic beverages. According to the invention, this process consists in resorting to two coupled reverse osmosis systems each comprising a tank for supplying the product to be processed upstream from a reverse osmosis cell, in equipping the two reverse osmosis cells P and I with membranes with a distinctly different permeability to ethanol, in withdrawing the permeate originating from said cell P, in sending it into said supply reservoir upstream from said cell I, in withdrawing the permeate originating from said cell I and in sending it into the supply tank upstream from cell P, whilst recovering the corresponding concentrates X and Y.

Abstract: The present invention relates to a process for treating fermented juices by reversed osmosis in particular for increasing concentration thereof in organic substances. The fermented product is treated under a pressure of from 30 to 120 bars and a strong turbulency by passing it through at least one semi-permeable membrane having a high retention rate with respect to organic substances of molecular weights lower than 200.

Abstract: In a known method of electrolytically marking metallic articles a hood having its bottom closed by a stencil with perforations is partially filled with an electrolyte and contains a cathode. An article to be marked is brought into contact with the stencil and constitutes the anode. The invention consists in maintaining the space within the hood at a partial vacuum sufficient to cause bubbles of air which have passed through the perforations in the stencil to flow upward in the hood, thus preventing electrolyte from seeping downward through said perforations.