Abstract: The present invention relates to a method for detecting an aggregate of an aggregate-forming polypeptide of a biosample, comprising (a) a step of spiking a dimer type of the aggregate-forming polypeptide into the biosample to be analyzed; (b) a step of incubating a resultant product of step (a) to further form an aggregate of the aggregate-forming polypeptide; (c) a step of contacting a resultant product of step (b) with a binding agent-tag in which a signal-generating tag is bound to a binding agent that binds to the aggregate of the aggregate-forming polypeptide; and (d) a step of detecting a signal which is generated from the binding agent-tag bound to the aggregate of the aggregate-forming polypeptide.

Abstract: The present invention relates to a method for detecting an aggregate of an aggregate-forming polypeptide of a biosample, comprising (a) a step of spiking a dimer type of the aggregate-forming polypeptide into the biosample to be analyzed; (b) a step of incubating a resultant product of step (a) to further form an aggregate of the aggregate-forming polypeptide; (c) a step of contacting a resultant product of step (b) with a binding agent-tag in which a signal-generating tag is bound to a binding agent that binds to the aggregate of the aggregate-forming polypeptide; and (d) a step of detecting a signal which is generated from the binding agent-tag bound to the aggregate of the aggregate-forming polypeptide

Abstract: The present invention relates to a method for detecting an aggregate form of an aggregate-forming polypeptide in a biosample, comprising the steps of: (a) spiking, in a biosample to be analyzed, (i) a monomeric or multimeric form of an aggregate-forming polypeptide, (ii) a hydrophobic deleted derivative of the aggregate-forming polypeptide, or (iii) a monomeric or multimeric form of the aggregate-forming polypeptide and a hydrophobic deleted derivative of the aggregate-forming polypeptide; (b) additionally forming the aggregate form of the aggregate-forming polypeptide by incubating the product of step (a); (c) making the product of step (b) come into contact with a binder-label in which a signal-generating label is coupled to a binder binding to the aggregate form of the aggregate-forming polypeptide; and (d) detecting a signal to be generated from the binder-label bound to the aggregate form of the aggregate-forming polypeptide.