Abstract: Provided is a vector capable of co-expressing two different target proteins on the microbial surface using two promoters derived from Lactobacillus, and a method of expressing target proteins on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism, and allows different foreign proteins to be stably expressed on the surface of the microorganism. Furthermore, provided is a surface expression vector containing the gene pgsA encoding a poly-gamma-glutamate synthetase complex, and a method of expressing a target protein on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism, and allows the foreign proteins to be stably expressed on the surface of the microorganism.

Abstract: Provided is a galactose mutarotase gene promoter derived from Lactobacillus casei and the use thereof, and more particularly, to a Lactobacillus casei-derived galactose mutarotase gene promoter having the nucleotide sequence of SEQ ID NO: 1, an expression vector containing the promoter, and a microorganism transformed with the expression vector. A microorganism transformed with an expression vector containing the promoter may effectively express a target protein on the cell surface, and thus is useful as a vaccine vehicle or the like. Moreover, provided is a surface expression vector having pgsA, which is a gene encoding poly-gamma-glutamate synthetase, and a method of expressing a target protein on the microbial surface using the vector. The vector containing foreign genes inserted therein is transformed into a microorganism and allows a foreign protein is to be stably expressed on the surface of the microorganism.

Abstract: The speaker comprises a ring-shaped frame 110, a magnetic circuit section 120, a vibration section 130, a terminal 140, a tinsel wire 145, and a weight ring 150. The ring-shaped frame 110 has a through hole formed in the center of thereof. The magnetic circuit section 120 is coupled to the outside of the through hole. The vibrating section 130 include a diaphragm 131 with an upper end coupled to the inner side of the through hole at the upper side of the frame 110, and a voice coil 133 having an upper end coupled to the diaphragm 131 and a lower end located in the magnetic circuit section 120. The tinsel wire 145 has one end connected to the terminal 140 and the other end connected to the voice coil 133. The weight ring 150 is coupled to the outer surface of the voice coil 133. With this configuration, the weight ring coupled to the voice coil allows the weight of the voice coil 133 to be increase.