Patents by Inventor H. D. Alan Lindquist

H. D. Alan Lindquist has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • In-situ hybridization probes for the detection of microsporidial species
    Patent number: 6855498
    Abstract: The present invention provides in situ hybridization probes which include a marker and a nucleic acid molecule able to hybridize exclusively with only one species of Encephalitozoon. The nucleic acid molecule may be, for example, complimentary to segment 878-896 of 16S rRNA of Encephalitozoon hellem spores. Specifically disclosed probes are those including the following nucleotides: (1) 5?-ACT CTC ACA CTC ACT TCA G-3? (Seq. I.D. No. 1) which is species specific for Encephalitozoon hellem, (2) 5?-CAG ACC ACT ATC TGC A-3? (Seq. I.D. No. 2) which is species specific for Encephalitozoon cuniculi and (3) 5?-GTT CTC CTG CCC GCT TCA G-3? (Seq. I.D. No. 3) which is species specific for Encephalitozoon intestinalis. The assay of the present invention utilizes a sample such as surface, ground or drinking water, suspected of containing one of the aforementioned species as a target organism.
    Type: Grant
    Filed: September 18, 2001
    Date of Patent: February 15, 2005
    Assignee: U.S. Environmental Protection Agency
    Inventors: Jeffery Dean Hester, H. D. Alan Lindquist, Frank W. Schaefer, III
  • Detection of microsporidial species using a quantitative real-time PCR assay
    Publication number: 20030215811
    Abstract: Microsporidial species can be detected in samples using real-time PCR dual-fluorescent assays with species-specific primer set and a dual fluorescent labeled hybridization probe. Cyclospora cayetanensis can be detected in samples using a real-time PCR dual-fluorescent assay with a primer set and dual fluorescent labeled hybridization probe.
    Type: Application
    Filed: May 17, 2002
    Publication date: November 20, 2003
    Inventors: Frank Schaefer, H.D. Alan Lindquist, Jeffery Dean Hester, Manju Varma
  • In-situ hybridization probes for the detection of microsporidial species
    Publication number: 20020102584
    Abstract: The present invention provides in situ hybridization probes which include a marker and a nucleic acid molecule able to hybridize exclusively with only one species of Encephalitozoon. The nucleic acid molecule may be, for example, complimentary to segment 878-896 of 16S rRNA of Encephalitozoon hellem spores. Specifically disclosed probes are those including the following nucleotides: (1) 5′-ACT CTC ACA CTC ACT TCA G-3′ (Seq. I.D. No. 1) which is species specific for Encephalitozoon hellem, (2) 5′-CAG ACC ACT ATC TGC A-3′ (Seq. I.D. No. 2) which is species specific for Encephalitozoon cuniculi and (3) 5′-GTT CTC CTG CCC GCT TCA G-3′ (Seq. I.D. No. 3) which is species specific for Encephalitozoon intestinalis. The assay of the present invention utilizes a sample such as surface, ground or drinking water, suspected of containing one of the aforementioned species as a target organism.
    Type: Application
    Filed: September 18, 2001
    Publication date: August 1, 2002
    Inventors: Jeffery Dean Hester, H.D. Alan Lindquist, Frank W. Schaefer