Patents by Inventor H. Garrett Wada

H. Garrett Wada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9766216
    Abstract: This invention provides methods and compositions, e.g., to reduce interference from non-specific binding sample constituents in a migration shift assay. Interference due to non-specific binding of sample constituents to an affinity substance (e.g., an affinity molecule or a conjugate of an affinity molecule and a charged carrier molecule) is prevented by, e.g., binding the constituents to charged polymers such as heparin sulfate. The present invention also provides methods to concentrate an analyte of interest with high concentration and to detect the analyte with high sensitivity, and further to optimize the reaction conditions for easily concentrating the analyte. Such objects of the present invention are attained, for example, by concentrating a complex of the analyte and a conjugate which is formed by contacting the analyte in a sample with an affinity molecule bound to a charged carrier molecule such as DNA.
    Type: Grant
    Filed: April 8, 2004
    Date of Patent: September 19, 2017
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: H. Garrett Wada, Irina G. Kazakova, Yutaka Miki, Toshinari Ohashi, Futoshi Kanke
  • Patent number: 8216852
    Abstract: The present invention provides novel methods for controlling/manipulating materials flowing in a fluidic device. In particular, the methods of the invention create and utilize differences between dispersion rates and/or average velocity of materials in order to manipulate the materials.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: July 10, 2012
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrea W. Chow, H. Garrett Wada
  • Publication number: 20110063943
    Abstract: The present invention provides novel methods for controlling/manipulating materials flowing in a fluidic device. In particular, the methods of the invention create and utilize differences between dispersion rates and/or average velocity of materials in order to manipulate the materials.
    Type: Application
    Filed: November 17, 2010
    Publication date: March 17, 2011
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Andrea W. Chow, H. Garrett Wada
  • Patent number: 7754150
    Abstract: Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
    Type: Grant
    Filed: July 2, 2003
    Date of Patent: July 13, 2010
    Assignee: Caliper Life Sciences, Inc.
    Inventors: H. Garrett Wada, Matthew B. Murphy
  • Patent number: 6989128
    Abstract: Apparatus and methods for modulating flow rates in microfluidic devices are provided. The methods involve modulating downstream pressure in the device to change the flow rate of materials in an upstream region of the device. Such methods include electrokinetic injection or withdrawal of materials through a side channel and the use of an absorbent material to induce wicking in the channel system. The apparatus provided includes a prefabricated wick in the device to provide for flow rate control. Additional methods for determining velocity of a particle and cell incubation time are also provided.
    Type: Grant
    Filed: May 8, 2002
    Date of Patent: January 24, 2006
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Marja Liisa Alajoki, H. Garrett Wada, Robert S. Dubrow
  • Patent number: 6979553
    Abstract: Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
    Type: Grant
    Filed: September 5, 2003
    Date of Patent: December 27, 2005
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Javier Anibal Farinas, H. Garrett Wada
  • Patent number: 6759191
    Abstract: Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
    Type: Grant
    Filed: January 21, 2003
    Date of Patent: July 6, 2004
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Javier Anibal Farinas, H. Garrett Wada
  • Publication number: 20040048299
    Abstract: Methods of monitoring transporter activity, gradient induced activity, and binding activity in microscale systems, as well as corresponding microscale devices, systems and kits are provided.
    Type: Application
    Filed: August 28, 2003
    Publication date: March 11, 2004
    Applicant: Caliper Technologies Corp.
    Inventors: J. Wallace Parce, C. Nicholas Hodge, H. Garrett Wada
  • Publication number: 20040048239
    Abstract: Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
    Type: Application
    Filed: September 5, 2003
    Publication date: March 11, 2004
    Applicant: Caliper Technologies Corp.
    Inventors: Javier Anibal Farinas, H. Garrett Wada
  • Publication number: 20040048360
    Abstract: Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
    Type: Application
    Filed: July 2, 2003
    Publication date: March 11, 2004
    Applicant: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Matthew B. Murphy
  • Publication number: 20040009545
    Abstract: Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
    Type: Application
    Filed: January 21, 2003
    Publication date: January 15, 2004
    Applicant: Caliper Technologies Corp.
    Inventors: Javier Anibal Farinas, H. Garrett Wada
  • Patent number: 6649358
    Abstract: Methods of monitoring transporter activity, gradient induced activity, and binding activity in microscale systems, as well as corresponding microscale devices, systems and kits are provided.
    Type: Grant
    Filed: May 25, 2000
    Date of Patent: November 18, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: J. Wallace Parce, C. Nicholas Hodge, H. Garrett Wada
  • Patent number: 6613581
    Abstract: Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
    Type: Grant
    Filed: August 17, 2000
    Date of Patent: September 2, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Matthew B. Murphy
  • Patent number: 6592821
    Abstract: Methods and systems for particle focusing to increase assay throughput and sensitivity in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
    Type: Grant
    Filed: May 10, 2001
    Date of Patent: July 15, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Anne R. Kopf-Sill, Marja Liisa Alajoki, J. Wallace Parce, Benjamin N. Wang, Andrea W. Chow, Robert S. Dubrow, Yevgeny Yurkovetsky, Javier Anibal Farinas
  • Patent number: 6537771
    Abstract: Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: March 25, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: Javier Anibal Farinas, H. Garrett Wada
  • Patent number: 6529835
    Abstract: Methods for determining a function of cells, which comprises a suspension of cells flowing along a first fluid channel. The cells have a first detectable property associated therewith, and wherein the cells produce a second detectable property upon activation of the function of the cells, the first and second detectable properties being distinguishable from each other. Levels of the first and second detectable properties are measured. The level of the second detectable property is compared to the level of first detectable property to determine the relative function of the cells.
    Type: Grant
    Filed: June 26, 2000
    Date of Patent: March 4, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Steven A. Sundberg
  • Publication number: 20030027225
    Abstract: The present invention provides systems, devices and methods for performing fast and efficient separation and collection of components of a sample mixtures. The present invention provides integrated systems for performing separation using a multi port control system to sort and collect components of sample mixtures.
    Type: Application
    Filed: July 12, 2002
    Publication date: February 6, 2003
    Applicant: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Luc J. Bousse, Andrea W. Chow
  • Patent number: 6506609
    Abstract: Methods and systems for particle focusing to increase assay throughput in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
    Type: Grant
    Filed: May 11, 2000
    Date of Patent: January 14, 2003
    Assignee: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Anne R. Kopf-Sill, Marja Liisa Alajoki, J. Wallace Parce, Benjamin N. Wang, Andrea W. Chow, Robert S. Dubrow
  • Publication number: 20020179445
    Abstract: Apparatus and methods for modulating flow rates in microfluidic devices are provided. The methods involve modulating downstream pressure in the device to change the flow rate of materials in an upstream region of the device. Such methods include electrokinetic injection or withdrawal of materials through a side channel and the use of an absorbent material to induce wicking in the channel system. The apparatus provided includes a prefabricated wick in the device to provide for flow rate control. Additional methods for determining velocity of a particle and cell incubation time are also provided.
    Type: Application
    Filed: May 8, 2002
    Publication date: December 5, 2002
    Applicant: Caliper Technologies Corp.
    Inventors: Marja Liisa Alajoki, H. Garrett Wada, Robert S. Dubrow
  • Publication number: 20020127591
    Abstract: Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby the second component is internalized within the biological cell. At least one of the first and second components has a fluorescent label. The amount of binding between the first and second components within the cell is determined by measuring a level of polarized and/or depolarized fluorescence emitted from within the biological cell.
    Type: Application
    Filed: March 5, 2002
    Publication date: September 12, 2002
    Applicant: Caliper Technologies Corp.
    Inventors: H. Garrett Wada, Javier A. Farinas, Theo T. Nikiforov