Abstract: The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.

Abstract: Apparatus and method for performing sequential bioassays with microfluidic transfers.

Abstract: The present teachings pertain to methods, reaction mixtures, and kits for ligating polynucleotides. In some embodiments, a heat-activatable ligation agent, a phosphorylation agent, and a decontamination agent are included in the same ligation reaction mixture with at least one probe set, at least one linker set, and at least one target polynucleotide. A reaction at a first temperature results in hybridization of the probes to the target, phosphorylation of the probes, and decontamination of unwanted reaction components. A reaction at a second temperature results in the ligation of the probes together. In some embodiments, the present teachings are applied in highly multi-plexed ligation reactions in which a plurality of single nucleotide polymorphisms in a plurality of target polynucleotides are queried, and eventually detected using a mobility dependent analysis technique.

Abstract: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample. In some embodiments of the present teachings, probes are hybridized to complementary target polynucleotides and are ligated together to form a ligation product. Some embodiments of the present teachings comprise positive assay control probes that provide information regarding the occurrence of specific ligation in a complex ligation assay mixture. Some embodiments of the present teachings provide for negative assay control probes that provide information regarding the occurrence of non-specific ligation in a complex ligation assay mixture. Some embodiments of the present teachings provide for the generation of two distinct signals from a monomorphic target polynucleotide sequence.