Patents by Inventor Hagan Bayley

Hagan Bayley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190093071
    Abstract: The present invention relates to a process for producing a droplet assembly, which droplet assembly comprises a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium, which process comprises: generating, in a bulk hydrophobic medium, a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium.
    Type: Application
    Filed: February 28, 2017
    Publication date: March 28, 2019
    Inventors: Hagan Bayley, Sam Olof, Alexander D. Graham, Madeline Burke, James Armstrong, Adam Perriman, James Nicholson, Francis Szele
  • Publication number: 20190062729
    Abstract: The present invention relates to activatable promoters, as well as expression systems, vectors, delivery systems, reaction vessels, aqueous objects and compositions comprising said activatable promoters. The invention also provides compositions comprising assemblies and networks of aqueous objects comprising said activatable promoters. The invention additionally provides methods of expressing a transcript and methods of expressing a polypeptide using the activatable promoters of the invention. The invention further provides phospholipid mixtures, as well as aqueous objects and compositions comprising said phospholipid mixtures.
    Type: Application
    Filed: February 28, 2017
    Publication date: February 28, 2019
    Inventors: Hagan Bayley, Michael Booth
  • Publication number: 20190024034
    Abstract: The present invention relates to a process for producing a composition comprising an aqueous medium and, disposed in the aqueous medium, a first volume of a first hydrogel, which process comprises: (i) providing a composition comprising a first hydrophobic medium and, disposed in the first hydrophobic medium, a first volume of a first hydrogel; (ii) disposing a volume of an aqueous composition comprising a hydrogel compound around the first volume of the first hydrogel; (iii) allowing the aqueous composition comprising the hydrogel compound to form a gel and thereby forming a hydrogel object, which hydrogel object comprises the first volume of the first hydrogel and a second volume of a second hydrogel, which second volume of the second hydrogel is disposed around the first volume of the first hydrogel; and (iv) transferring the hydrogel object from the first hydrophobic medium to an aqueous medium and thereby producing the composition comprising the aqueous medium and, disposed in the aqueous medium, the fir
    Type: Application
    Filed: February 28, 2017
    Publication date: January 24, 2019
    Inventors: Hagan Bayley, Sam Olof, Alexander D. Graham
  • Patent number: 9963518
    Abstract: Compounds which inhibit Wza-mcdiated polysaccharide transport are useful in the prevention or treatment of bacterial infection, in particular of E. coli infection. The compounds are typically cyclic oligosaccharides such as cyclodextrins, which bear positively charged functional groups at the primary carbon position.
    Type: Grant
    Filed: August 20, 2012
    Date of Patent: May 8, 2018
    Assignee: Oxford University Innovation Limited
    Inventors: Lingbing Kong, Benjamin G. Davis, Hagan Bayley
  • Publication number: 20170226578
    Abstract: The invention relates to a method for detection of analyte interaction with a channel molecule held in a membrane, comprising the optical detection of a modification in the flux of a signal molecule as it passes through the channel molecule by the action of a membrane potential, wherein the modification in the flux is caused by at least partial blockage of the channel molecule by the analyte. The invention further relates to bilayer arrays, components, methods of manufacture and use.
    Type: Application
    Filed: July 9, 2015
    Publication date: August 10, 2017
    Applicant: OXFORD UNIVERSITY INNOVATION LIMITED
    Inventors: Mark WALLACE, Hagan BAYLEY, Shuo HUANG, Oliver Kieran CASTELL, Mercedes ROMERO-RUIZ
  • Publication number: 20160209317
    Abstract: The invention relates to a new method of determining the presence, absence, number or position(s) of one or more post-translational modifications in a peptide, polypeptide or protein. The invention uses transmembrane pores.
    Type: Application
    Filed: September 22, 2014
    Publication date: July 21, 2016
    Inventors: Hagan BAYLEY, David RODRIGUEZ-LARREA, Christian Bech ROSEN
  • Publication number: 20140296180
    Abstract: Compounds which inhibit Wza-mcdiated polysaccharide transport are useful in the prevention or treatment of bacterial infection, in particular of E. coli infection. The compounds are typically cyclic oligosaccharides such as cyclodextrins, which bear positively charged functional groups at the primary carbon position.
    Type: Application
    Filed: August 20, 2012
    Publication date: October 2, 2014
    Applicant: ISIS INNOVATION LIMITED
    Inventors: Lingbing Kong, Benjamin G. Davis, Hagan Bayley
  • Patent number: 8822160
    Abstract: The invention relates to transmembrane protein pore for use in detecting a analyte in a sample. The pore comprises a molecular adaptor that facilitates an interaction between the pore and the analyte. The adaptor is covalently attached to the pore in an orientation that allows the analyte to be detected using the pore.
    Type: Grant
    Filed: October 6, 2008
    Date of Patent: September 2, 2014
    Assignee: Isis Innovation Limited
    Inventors: John Hagan Bayley, Haichen Wu, Giovanni Maglia, Yann Astier
  • Patent number: 8785211
    Abstract: The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
    Type: Grant
    Filed: December 28, 2011
    Date of Patent: July 22, 2014
    Assignee: Isis Innovation Limited
    Inventors: Hagan Bayley, Yann Astier, Orit Braha
  • Patent number: 8404449
    Abstract: A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or ?HL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: March 26, 2013
    Assignee: The Texas A&M University System
    Inventors: Hagan Bayley, Seong-Ho Shin, Tudor Luchian, Stephen Cheley
  • Publication number: 20120219960
    Abstract: The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
    Type: Application
    Filed: December 28, 2011
    Publication date: August 30, 2012
    Applicant: ISIS INNOVATION LIMITED
    Inventors: Hagan BAYLEY, Yann ASTIER, Orit BRAHA
  • Patent number: 8105846
    Abstract: The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
    Type: Grant
    Filed: November 15, 2006
    Date of Patent: January 31, 2012
    Assignee: Isis Innovation Limited
    Inventors: Hagan Bayley, Yann Astier, Orit Braha
  • Publication number: 20110229877
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: July 6, 2009
    Publication date: September 22, 2011
    Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED
    Inventors: Lakmal Jayasinghe, Hagan Bayley, Stephen Cheley, Brian Mckeown, James White, James Clarke
  • Publication number: 20110177498
    Abstract: The invention relates to a mutant ?-hemolysin (?-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment.
    Type: Application
    Filed: July 6, 2009
    Publication date: July 21, 2011
    Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED
    Inventors: James Clarke, Lakmal Jayasinghe, Terence Reid, Hagan Bayley
  • Patent number: 7867716
    Abstract: The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55° C. and even as high as near 100° C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.
    Type: Grant
    Filed: December 21, 2005
    Date of Patent: January 11, 2011
    Assignee: The Texas A&M University System
    Inventors: Xiaofeng Kang, Li Qun Gu, Stephen Cheley, Hagan Bayley
  • Publication number: 20100297638
    Abstract: The invention relates to transmembrane protein pore for use in detecting a analyte in a sample. The pore comprises a molecular adaptor that facilitates an interaction between the pore and the analyte. The adaptor is covalently attached to the pore in an orientation that allows the analyte to be detected using the pore.
    Type: Application
    Filed: October 6, 2008
    Publication date: November 25, 2010
    Applicant: ISIS INNOVATION LIMITED
    Inventors: John Hagan Bayley, Haichen Wu, Giovanni Maglia, Yann Astier
  • Publication number: 20090283422
    Abstract: A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered PSH type or ?HL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.
    Type: Application
    Filed: April 24, 2009
    Publication date: November 19, 2009
    Inventors: Hagan Bayley, Seong-Ho Shin, Tudor Luchian, Stephen Cheley
  • Publication number: 20080311582
    Abstract: The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.
    Type: Application
    Filed: November 15, 2006
    Publication date: December 18, 2008
    Inventors: Hagan Bayley, Yann Astier, Orit Braha
  • Publication number: 20080101988
    Abstract: The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55° C. and even as high as near 100° C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.
    Type: Application
    Filed: December 21, 2005
    Publication date: May 1, 2008
    Applicant: The Texas A&M University System
    Inventors: Xiaofeng Kang, Li Qun Gu, Stephen Cheley, Hagan Bayley
  • Publication number: 20070298511
    Abstract: The invention relates to a nanopore sensor system including methods of fabrication and uses disclosed herein. In some embodiments, the invention relates to a substrate comprising a lipid membrane, preferably a phospholipid bilayer film, having a nanopore and a gel surrounding said lipid membrane. In additional embodiments, the invention relates to compositions and methods of using and making a substrate that has a lipid membrane having a single channel protein surrounded with a gel. In further embodiments, the invention relates to a method of detecting an analyte by mixing a nanopore sensor with a solution suspected of containing an analyte, measuring electrical properties, and correlating changes of electrical properties to the existence of an analyte.
    Type: Application
    Filed: April 19, 2007
    Publication date: December 27, 2007
    Inventors: Xiaofeng Kang, Stephen Cheley, Hagan Bayley