Patents by Inventor Hai-Quan Mao

Hai-Quan Mao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240084324
    Abstract: Disclosed are the optimal composition and size of DNA/polycation particles for efficient transfection of viral production cells in both adherent and suspension cultures. The size-dependent feature of DNA/polycation particle-mediated transfection for particles between 50 nm and 1000 nm also is disclosed. A new scalable method based on kinetic control of DNA/polycation nanoparticle assembly to prepare shelf-stable particles with defined sizes between 50 nm and 1000 nm also is disclosed. The presently disclosed DNA/polycation particles yield superior and reproducible transfection activity and shelf stability and can be used as an off-the-shelf product.
    Type: Application
    Filed: February 16, 2022
    Publication date: March 14, 2024
    Inventors: Hai-Quan Mao, Yizong Hu, Yining Zhu
  • Publication number: 20240042430
    Abstract: A device for selectively capturing mycobacteria comprises a substrate and a capture polymer layer of poly-diallyldimethyl ammonium chloride, wherein the capture polymer layer is covalently linked onto the substrate via a UV-initiated polymerization reaction of a solution comprising diallyldimethyl ammonium chloride and a photoinitiator in water purged of dissolved oxygen, and wherein the UV exposure time is 30 seconds to 4 minutes at a power density of about 20 to about 25 mW/cm2. A kit can comprise the device. A microfluidic chip comprises at least a portion of at least one channel sidewall coated with a capture polymer layer of poly-diallyldimethyl ammonium chloride. A method for manufacturing the device includes plasma treating a substrate, providing a solution comprising diallyldimethyl ammonium chloride and a photoinitiator in water purged of dissolved oxygen, and coating the plasma-treated substrate via a UV-initiated polymerization reaction.
    Type: Application
    Filed: January 22, 2022
    Publication date: February 8, 2024
    Inventors: Digvijay Singh, Bonolo Mathekga, Yukari Manabe, Soumyadipta Acharya, Hai Quan Mao
  • Publication number: 20240042103
    Abstract: Described are methods for embedding one or more therapeutic agents into vascular grafts and other scaffold-based devices, and methods of implanting vascular grafts comprising tubular scaffolds into subjects. The tubular scaffolds comprise hydrogel nanofibers that have internally aligned polymer chains and may contain one or more therapeutic agents.
    Type: Application
    Filed: December 13, 2021
    Publication date: February 8, 2024
    Inventors: Sharon Gerecht, Morgan B. Elliott, Hai-Quan Mao, Theresa Chen, Khyati Prasad
  • Publication number: 20240000905
    Abstract: Nanoparticles or microgels comprising a polyelectrolyte nanocomplex comprising one or more neuromodulators, a carrier molecule, and a counter ion polymer, wherein the counter ion polymer has a charge enabling it to bind electrostatically to the one or more neuromodulators, methods of their preparation, and methods of treating a disease or condition are disclosed.
    Type: Application
    Filed: November 30, 2021
    Publication date: January 4, 2024
    Inventors: Sashank Reddy, Hai-Quan Mao, Chenhu Qiu
  • Publication number: 20230405189
    Abstract: A composite material can include a gel and at least one nanostructure disposed within the gel. A method for healing a soft tissue defect can include applying a composite material to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a composite material for use in healing soft tissue defects can include providing a gel and disposing nanofibers within the gel.
    Type: Application
    Filed: June 14, 2023
    Publication date: December 21, 2023
    Inventors: Xuesong Jiang, Sashank Reddy, Gerald Brandacher, Hai-Quan Mao, Justin Sacks, Xiaowei Li, Kevin Feng, Russell Martin, Georgia C. Yalanis, Ji Suk Choi
  • Publication number: 20230389926
    Abstract: Biodegradable nanofiber conical conduits for nerve repair and methods of using same are disclosed. The biodegradable nanofiber conical conduits for nerve repair and methods provide a saturable conduit having a conical shape/geometry including a larger proximal aperture and smaller distal aperture to mechanically guide the regenerating axons across the mismatched repair and thereby prevent axonal escape and neuroma formation. The biodegradable nanofiber conical conduits for nerve repair may include, but are not limited to, a conical conduit that tapers substantially linearly; a conical conduit including a conical concave shape, a conical conduit including a conical convex shape, a conical conduit including proximal and/or distal extensions, a conical conduit including an arrangement of lateral or radial ridges for crimping action, and a conical conduit filled with hydrogel for inhibiting excessive axonal growth.
    Type: Application
    Filed: October 14, 2021
    Publication date: December 7, 2023
    Inventors: Hai-Quan Mao, Sami Tuffaha, Ahmet Hoke, Michael James Lan, Bruce Peter Enzmann, Anson Y. Zhou, Alison L. Wong, Erica Lee, Sai Pinni
  • Publication number: 20230338612
    Abstract: The presently disclosed composition and methods are provided for an in situ forming nanofiber-hydrogel composite, which is formed using non-covalent binding schemes between the fiber surface and hydrogel-forming polymers. A method for healing a soft tissue defect can include applying the said composite material to a soft tissue defect.
    Type: Application
    Filed: July 26, 2022
    Publication date: October 26, 2023
    Inventors: Russell Martin, Hai-quan Mao
  • Patent number: 11779682
    Abstract: The presently disclosed subject matter provides a scalable and electrostretching approach for generating hydrogel microfibers exhibiting uniaxial alignment from aqueous polymer solutions. Such hydrogel microfibers can be generated from a variety of water-soluble natural polymers or synthetic polymers. The hydrogel microfibers can be used for controlled release of bioactive agents. The internal uniaxial alignment exhibited by the presently disclosed hydrogel fibers provides improved mechanical properties to hydrogel microfibers, and contact guidance cues and induces alignment for cells seeded on or within the hydrogel microfibers.
    Type: Grant
    Filed: October 5, 2018
    Date of Patent: October 10, 2023
    Assignee: The Johns Hopkins University
    Inventors: Sharon Gerecht, Shuming Zhang, Sebastian F. Barreto Ortiz, Hai-Quan Mao
  • Patent number: 11771807
    Abstract: A soft tissue device can incorporate a composite material comprising a gel and at least one nanostructure disposed within the gel. A soft tissue device can further incorporate biologically active materials such as cells, tissues. A method for healing a soft tissue defect while promoting soft tissue regeneration can include applying a soft tissue device to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a soft tissue device for use in healing soft tissue defects can include providing a gel, disposing nanofibers within the gel, and a biologically active material.
    Type: Grant
    Filed: July 18, 2019
    Date of Patent: October 3, 2023
    Assignee: The Johns Hopkins University
    Inventors: Sashank Reddy, Russell Martin, Xiaowei Li, Calvin Chang, Kevin Colbert, Hai-Quan Mao
  • Publication number: 20230293776
    Abstract: A composite material can include a gel and at least one nanostructure disposed within the gel. A method for healing a soft tissue defect can include applying a composite material to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a composite material for use in healing soft tissue defects can include providing a gel and disposing nanofibers within the gel.
    Type: Application
    Filed: May 22, 2023
    Publication date: September 21, 2023
    Inventors: Xuesong Jiang, Sashank Reddy, Gerald Brandacher, Hai-Quan Mao, Justin Sacks, Xiaowei Li, Kevin Feng, Russell Martin, Georgia C. Yalanis, Ji Suk Choi
  • Publication number: 20230270919
    Abstract: Described are methods of making vascular grafts from man-made tubular scaffolds, tubular scaffolds, and methods implanting vascular grafts comprising tubular scaffolds into subjects. The tubular scaffolds of the present invention are made of hydrogel nanofibers that have internally aligned polymer chains and may be cellularized.
    Type: Application
    Filed: October 15, 2020
    Publication date: August 31, 2023
    Inventors: Sharon Gerecht, Morgan B. Elliott, Hai - Quan Mao, Jessica Shen
  • Publication number: 20230243825
    Abstract: Disclosed are methods for enriching and expanding antigen-specific T cells with paramagnetic nanoparticles comprising a major histocompatibility complex (MHC) or human leukocyte antigen (HLA) and a costimulatory molecule bound thereto. The platform eliminates the requirement of cell isolation and can be further adapted to be high throughput with the capability of processing multiple antigen-specific T cells in parallel. Accordingly, the disclosed methods provide a high-throughput workflow for the identification and analysis of antigen-specific T cell responses.
    Type: Application
    Filed: June 23, 2021
    Publication date: August 3, 2023
    Inventors: Jonathan Schneck, Hai-Quan Mao, John Wirthlin Hickey, Ariel Isser
  • Patent number: 11707553
    Abstract: A composite material can include a gel and at least one nanostructure disposed within the gel. A method for healing a soft tissue defect can include applying a composite material to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a composite material for use in healing soft tissue defects can include providing a gel and disposing nanofibers within the gel.
    Type: Grant
    Filed: August 6, 2019
    Date of Patent: July 25, 2023
    Assignee: The Johns Hopkins University
    Inventors: Xuesong Jiang, Sashank Reddy, Gerald Brandacher, Hai-Quan Mao, Justin Sacks, Xiaowei Li, Kevin Feng, Russell Martin, Georgia C. Yalanis, Ji Suk Choi
  • Publication number: 20230219044
    Abstract: A process and a device for continuous micromixing of two fluids is disclosed. The process and the device can be used in particular when micromixing plays an important role, for example, in the yield and characteristics of the products. This is the case for crystallization, precipitation and combustion reactions and for micelle assembly or polyelectrolyte complexation processes.
    Type: Application
    Filed: June 10, 2021
    Publication date: July 13, 2023
    Inventors: Rajat Mittal, Jung Hee Seo, Hai-Quan Mao, Yue Hao, Yizong Hu
  • Patent number: 11684700
    Abstract: A composite material can include a gel and at least one nanostructure disposed within the gel. A method for healing a soft tissue defect can include applying a composite material to a soft tissue defect, wherein the composite material includes a gel and a nanostructure disposed within the gel. A method for manufacturing a composite material for use in healing soft tissue defects can include providing a gel and disposing nanofibers within the gel.
    Type: Grant
    Filed: June 23, 2022
    Date of Patent: June 27, 2023
    Assignee: The Johns Hopkins University
    Inventors: Xuesong Jiang, Sashank Reddy, Gerald Brandacher, Hai-Quan Mao, Justin Sacks, Xiaowei Li, Kevin Feng, Russell Martin, Georgia C. Yalanis, Ji Suk Choi
  • Publication number: 20230190665
    Abstract: The present invention belongs to the technical field of nanomedicine, and relates to a method for preparing a therapeutic protein-loaded nanoparticle, as well as a therapeutic protein-loaded nanoparticle, a suspension and a pharmaceutical composition comprising the nanoparticle, and a pharmaceutical preparation comprising the nanoparticle, the suspension or the pharmaceutical composition. The present invention further relates to a use of the nanoparticle in manufacture of a pharmaceutical composition, wherein the pharmaceutical composition is useful in prevention or treatment of a disease that can be prevented or treated by the therapeutic protein comprised in the nanoparticle.
    Type: Application
    Filed: January 19, 2017
    Publication date: June 22, 2023
    Inventors: Hai-Quan Mao, Jose Luis Santos, Zhiyu He, Huahua Huang, Lixin Liu, Kam W. Leong, Yongming Chen
  • Publication number: 20230190667
    Abstract: The presently disclosed subject matter provides methods for continuously generating uniform polyelectrolyte complex (PEC) nanoparticles comprising: flowing a first stream comprising one or more water-soluble polycationic polymers at a first variable flow rate into a confined chamber; flowing a second stream comprising one or more water-soluble polyanionic polymers at a second variable flow rate into the confined chamber; and impinging the first stream and the second stream in the confined chamber until the Reynolds number is from about 1,000 to about 20,000, thereby causing the one or more water-soluble polycationic polymers and the one or more water-soluble polyanionic polymers to undergo a polyelectrolyte complexation process that continuously generates PEC nanoparticles. Compositions produced from the presently disclosed methods and a device for producing the compositions are also disclosed.
    Type: Application
    Filed: February 7, 2023
    Publication date: June 22, 2023
    Inventors: Hai-Quan Mao, Jose Luis Santos, Yong Ren, John-Michael Williford
  • Publication number: 20230083448
    Abstract: An aluminum nanoparticle adjuvant carrier system with stabilizing surface coatings that can efficiently deliver protein or nucleic acid antigen payloads to naive, resident APCs is disclosed.
    Type: Application
    Filed: February 5, 2021
    Publication date: March 16, 2023
    Inventors: Hai-Quan Mao, Gregory P. Howard
  • Publication number: 20230080504
    Abstract: Embolization compositions and methods for controlling undesired bleeding and other treatments are provided. Preferred composition may comprise (a) a crosslinked hydrogel material; and (b) a fiber material, wherein the composition comprises a plurality of macropores; and the hydrogel material and fiber material are bonded by covalent and/or non-covalent bonds.
    Type: Application
    Filed: January 15, 2021
    Publication date: March 16, 2023
    Inventors: Christos Georgiades, Shin-Jae Lee, Iyer Siddharth, Mathias Insley, Xingyu Jasmine Hu, Da In Diane Lee, Hai-Quan Mao
  • Publication number: 20230074885
    Abstract: The presently disclosed subject matter provides nanoparticles comprising bortezomib encapsulated in a non-water-soluble polymer matrix in a form of a bortezomib-tannic acid complex; methods for preparing the nanoparticle; and use of the nanoparticles for treating liver cancer.
    Type: Application
    Filed: February 5, 2021
    Publication date: March 9, 2023
    Inventors: Florin M. Selaru, Hai-Quan Mao, Ling Li, Yang Zhou, Zhiyu He, Kuntao Chen