Abstract: The present application provides methods and systems for identifying host cell protein impurities which have enzymatic activities in biopharmaceutical products and in samples during manufacturing processes. The present application provides various activity-based probes to characterize different enzyme classes of host cell protein impurities including probes containing functionalized molecules with reporter or affinity-based tags.

Abstract: The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

Abstract: The disclosure provides a respirator, which includes a ventilation device, configured for providing ventilation airflow to a patient. The respirator further includes a first monitor, configured for monitoring a pressure or a flow velocity with which the ventilation device ventilates the patient, and a second monitor, configured for measuring a pressure change that reflects a self-respiratory effort of the patient. The respirator also includes a processor, configured for identifying an inspiratory trigger moment or an expiratory trigger moment of the patient according to the pressure change that reflects the self-respiratory effort of the patient.

Abstract: The present invention provides methods and systems for isotyping and quantification of antibodies based on immunocapture and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. These antibodies are induced by the administration of pharmaceutical products. The immunocapture method comprises contacting samples with a solid support, wherein the pharmaceutical product has been cross-linked directly to the solid support. The MS analysis includes conducting peptide mapping, selecting unique peptides and fragment ions to generate MRM (multiple reaction monitoring) transitions, optimizing collision energy, and determining a LLOQ (lower limit of quantification).

Abstract: Methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample. The methods including: contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments; separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis; eluting separated antibody fragments from the one or more capillaries; and determining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest.

Abstract: Compositions and methods for identifying free thiols in protein are provided. An exemplary method labeling peptides with a tag to identify free thiols and a tag to identify native disulfide bonds and analyzing the tags using targeted MS2. In one embodiment, the method provides complete coverage of all 32 cysteine residues in an IgG molecule. In other embodiments the method covers the 16 cysteine residues on the heavy and light chains in an IgG molecule. In another embodiment, the method covers the 5 cysteine residues on each light chain of an IgG molecule. In another embodiment, the method covers the 11 cysteine residues on each heavy chain of an IgG molecule.

Abstract: The present disclosure provides methods and compositions for the determining the abundance and/or concentration of protein biomarkers in a biological sample.

Abstract: The present invention provides methods to quickly and efficiently assess the effect of antibody variants, including PTMs, e.g., glycosylation, oxidation, and deamidation variants, on the binding between the antibody and FcRn. In particular, the present invention is based, at least in part, on the development of an online, two dimension liquid chromatography (2D-LC) method for assessing binding between antibody variants and FcRn. In one aspect, the online 2D-LC is coupled with mass spectrometry (MS). The present invention allows for differentiation of antibody variants by peak pattern and retention time profile by affinity chromatography, and identification of these variants by mass spectrometry analysis.

Abstract: A printing control method includes the following steps: dividing the printing process of the printer into different working processes, obtaining the state information of the printer of different working processes in real time, and controlling the corresponding printing process on the basis of the obtained state information of the printer of different working processes. In addition, a printing control apparatus includes the following units: a unit for dividing the process, a unit for obtaining the state of the printer and a unit is for controlling printing process. The invention can make sure that every par data needed to be printed could be fully printed on the surface in order to realize the printing of the effective par data.

Abstract: A printing control method includes the following steps: dividing the printing process of the printer into different working processes, obtaining the state information of the printer of different working processes in real time, and controlling the corresponding printing process on the basis of the obtained state information of the printer of different working processes. In addition, a printing control apparatus includes the following units: a unit for dividing the process, a unit for obtaining the state of the printer and a unit is for controlling printing process. The invention can make sure that every par data needed to be printed could be fully printed on the surface in order to realize the printing of the effective par data.