Abstract: For in mold spraying, a double-inclined mixing nozzle is connected obliquely and fixedly with a side surface of a third half-mold through a lateral sealing structure and connected with a side surface of a first half-mold in an inclined and sealing manner, side faces of a first half-mold and the third half-mold are respectively provided with installation inclined surfaces. A lateral sealing structure includes a mounting plate and a sealing member. The sealing member is sleeved on the double-inclined mixing nozzle and is in transition fit with the double-inclined mixing nozzle and the mounting plate respectively. A butt sealing groove provided on the installation inclined surface of the first half-mold and is in sealing fit with the mounting plate.

Abstract: For in mold spraying, a double-inclined mixing nozzle is connected obliquely and fixedly with a side surface of a third half-mold through a lateral sealing structure and connected with a side surface of a first half-mold in an inclined and sealing manner, side faces of a first half-mold and the third half-mold are respectively provided with installation inclined surfaces. A lateral sealing structure includes a mounting plate and a sealing member. The sealing member is sleeved on the double-inclined mixing nozzle and is in transition fit with the double-inclined mixing nozzle and the mounting plate respectively. A butt sealing groove provided on the installation inclined surface of the first half-mold and is in sealing fit with the mounting plate.

Abstract: A real time and quantitative method of measuring traction force of living cells include the following procedures. Place AT-cut and BT-cut quartz crystals of the same frequency, surface morphology and/or modified with the same cell adhesion molecules in petri dishes or detection cells; add the cells to the petri dishes or detection cells, the cell traction force at arbitrary time t during adhesion of the cells or under different internal/external environmental stimulations is estimated by the following equation: ?St=(KAT?KBT)?1[tqAT?ftAT/frAT?tqBT?ftBT/frBT]. The method can be used to track the dynamic changes of cells generated force during the adhesion of cells and under different internal/external environmental stimulations, such as the effects of drugs. The drugs can be added before or after the adhesion of the cells. This method is suitable for all adherent cells, including primary cells and passage cells.

Abstract: A real time and quantitative method of measuring traction force of living cells include the following procedures. Place AT-cut and BT-cut quartz crystals of the same frequency, surface morphology and/or modified with the same cell adhesion molecules in petri dishes or detection cells; add the cells to the petri dishes or detection cells, the cell traction force at arbitrary time t during adhesion of the cells or under different internal/external environmental stimulations is estimated by the following equation: ?St=(KAT?KBT)?1[tqAT?ftAT/frAT?tqBT?ftBT/frBT]. The method can be used to track the dynamic changes of cells generated force during the adhesion of cells and under different internal/external environmental stimulations, such as the effects of drugs. The drugs can be added before or after the adhesion of the cells. This method is suitable for all adherent cells, including primary cells and passage cells.