Abstract: A method to correct amplification bias in amplicon sequencing is disclosed. Amplification efficiency is not constant among different loci in a sample, nor for the same locus in different samples. Differences in 3?-end stability, primer Tm, amplicon length, amplicon GC content, and GC content of amplicon flanking regions all may contribute to amplification bias. Such bias interferes with accurate calculation of copy number for a genomic region of interest and hinders the application of amplicon sequencing for detection of minor copy number variation. The methods of the invention allow correction of amplification bias and enable detection of minor copy number variation using amplicon sequence data.

Abstract: An image preprocessing module is provided by the present invention to pre-process pathological images through superpixel segmentation in the cloud infrastructure, and meanwhile stores the boundary thereof by using the binary image; and performing multi-user data recording by the data recording module, so that the user can collaborate on image annotation; through the front-end interaction module, the capturing action of the user and displaying the pathological image; through the back-end processing module, the responding to the request sent by the front-end interaction module and completing the annotation of the data and the suggesting possible annotation sub-regions to the users. Through the effects achieved by the above modules, the present invention accomplishes the purpose of multi-user collaborative and convenient pixel-level annotation on pathological images via a webpage.

Abstract: A method to correct amplification bias in amplicon sequencing is disclosed. Amplification efficiency is not constant among different loci in a sample, nor for the same locus in different samples. Differences in 3?-end stability, primer Tm, amplicon length, amplicon GC content, and GC content of amplicon flanking regions all may contribute to amplification bias. Such bias interferes with accurate calculation of copy number for a genomic region of interest and hinders the application of amplicon sequencing for detection of minor copy number variation. The methods of the invention allow correction of amplification bias and enable detection of minor copy number variation using amplicon sequence data.

Abstract: The present invention relates to methods and compositions for preparing sequencing libraries. The methods and compositions provided herein enables next generation sequencing library preparation using multiplex PCR with reduced primer dimer formation.

Abstract: A method for automatically converting electronic data is disclosed.

Abstract: An image preprocessing module is provided by the present invention to pre-process pathological images through superpixel segmentation in the cloud infrastructure, and meanwhile stores the boundary thereof by using the binary image; and performing multi-user data recording by the data recording module, so that the user can collaborate on image annotation; through the front-end interaction module, the capturing action of the user and displaying the pathological image; through the back-end processing module, the responding to the request sent by the front-end interaction module and completing the annotation of the data and the suggesting possible annotation sub-regions to the users. Through the effects achieved by the above modules, the present invention accomplishes the purpose of multi-user collaborative and convenient pixel-level annotation on pathological images via a webpage.

Abstract: Disclosed are methods and compositions for amplification of genetic material, including isothermal WGA of single cells.

Abstract: Disclosed are methods and genotyping panels for detecting alleles, genomes, and transcriptomes in admixtures of two individuals.

Abstract: A method for automatically converting electronic data is disclosed.

Abstract: An excess heat recovery apparatus and process for high temperature chlorine dioxide bleaching of pulp is provided. The pulp of the high temperature chlorine dioxide bleaching stage enters a tube-side of a chlorine dioxide preheater through a pipeline. The low-temperature chlorine dioxide in the storage tank enters a shell-side pipeline of the chlorine dioxide preheater. 0.5 mol/L of a stabilizer may be added during preheating to prevent ClO2 from decomposing during the heating process. Preheated chlorine dioxide is then moved into a pulp mixer and the pH is adjusted to 3.2-3.8. The mixed pulp is then moved into a high temperature chlorine dioxide bleaching tower for bleaching. The cooling pulp, now out of the preheater, is washed in an alkaline extraction stage. The waste water from the washing flows directly into an effluent treatment system and is recycled after treatment.

Abstract: Disclosed are methods and genotyping panels for detecting alleles, genomes, and transcriptomes in admixtures of two individuals.

Abstract: Materials and methods for processing a cell population, such as enriching for a cell of interest from a cell population, are disclosed. The method may include: obtaining a cell population dispersed in a cytocompatible matrix; applying one or more labels to the cell population to distinguish a cell of interest from one or more cells of the cell population; identifying a portion of the cytocompatible matrix containing the cell of interest; and optionally isolating the portion of the cytocompatible matrix containing the cell of interest. The methods may, for example, advantageously provide reduced cell damage or loss relative to other procedures. Also disclosed are devices and compositions that may be useful for performing the methods.

Abstract: Disclosed are methods and genotyping panels for detecting alleles, genomes, and transcriptomes in admixtures of two individuals.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on a switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format. Optical and/or fluidic switching forces are used alone or in combination to effect switching.

Abstract: Systems, apparatus and methods are provided for biochemical analysis of a sample (e.g., a cell or nucleic acids). Samples are analyzed for molecular information and remain accessible for subsequent analysis or testing. The systems, apparatus and methods and systems provided are useful for performing quantitative and highly parallel biochemical reactions on biological samples in a high-throughput manner.

Abstract: Disclosed are methods and compositions for amplification of genetic material, including isothermal WGA of single cells.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on a switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format. Optical and/or fluidic switching forces are used alone or in combination to effect switching.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on an optical switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format.

Abstract: Described herein are compositions and methods useful for the detection of nucleic acid variations. Ligation within a probe or between probes is used to distinguish between probes perfectly complementary to a target and those containing a mismatch. Nucleotide fill-in/extension steps are optionally applied according to the type of assay performed. A circularization and relinearization step can be applied to create a template for further amplification and detection. In certain aspects, portions of a target sequence or its complement are not amplified.

Abstract: Disclosed are methods and genotyping panels for detecting alleles, genomes, and transcriptomes in admixtures of two individuals.