Abstract: A cell model for in vitro evaluation of compound-induced skin sensitization and a constructing method therefor. The method for constructing the cell model comprises the steps of: designing and constructing an sgRNA expression vector based on CRISPR/Cas9 vector system; designing and constructing a homologous recombinant vector capable of knocking a reporter gene linked to a self-cleaving peptide sequence into a specific site of the expression frame of the HMOX1 gene; co-transfecting the homologous recombinant vector, an hCas9 plasmid and the sgRNA expression vector into a cell, and carrying out monoclonal expansion to obtain the cell model. The present invention obtains a HaCaT cell model in which a luciferase gene is knocked in before the stop codon of the HMOX1 gene by combination of CRISPR/CAS9 and a monoclonal cell technique. The cell model realizes synchronous expression of the luciferase gene and the HMOX1 gene, thereby effectively distinguishing sensitizing compounds from non-sensitizing compounds.

Abstract: A method receives a first request from a client object at a device. The first request specifies a data source. In response to the first request, the method uploads data from the data source, stores the data as a plurality of first columns, and instantiates a first server object that provides access to the first columns. The method later receives a second request from the client object. The second request specifies a transformation of the data. In response to the second request, the method stores one or more additional columns and instantiates a second server object that provides access to the additional columns and one or more of the first columns. Each of the additional columns is constructed from the first columns according to the requested transformation, and each of the additional columns includes a plurality of data values all having the same data type.

Abstract: Methods, systems, and computer readable media for session based software defined networking (SDN) management are disclosed. According to one method, the method occurs at a policy engine. The method includes determining, using a session identifier, Third Generation Partnership Project (3GPP) session information associated with a user device. The method also includes generating, using the 3GPP session information, SDN policy information for SDN related traffic associated with the 3GPP session, wherein the SDN policy information is for providing a quality of service (QoS) for the SDN related traffic corresponding to a QoS associated with the 3GPP session information, wherein the SDN related traffic is correlated with the 3GPP session using a source address associated with the user device.

Abstract: Methods, systems, and computer readable media for session based software defined networking (SDN) managment are disclosed. According to one method, the method occurs at a policy engine. The method includes determining, using a session identifier, Third Generation Partnership Project (3GPP) session information associated with a user device. The method also includes generating, using the 3GPP session information, SDN policy information for SDN related traffic associated with the 3GPP session, wherein the SDN policy information is for providing a quality of service (QoS) for the SDN related traffic corresponding to a QoS associated with the 3GPP session information, wherein the SDN related traffic is correlated with the 3GPP session using a source address associated with the user device.