Abstract: The present invention provides a method for generating a droplet array on a microfluidic chip, comprising the following steps: assembling an upper chip and a lower chip to an initial position, a fluid channel of the upper chip partially or completely covering a microporous array of the lower chip; injecting a solution into a chip, the solution being partially or fully filled in the microporous array of the lower chip; and relatively moving the upper chip and the lower chip to a liquid dividing position, the fluid channel of the upper chip and the microporous array of the lower chip being overlapped no longer, and the solution being dispersed into the microporous array to form a droplet array. A contact surface between the upper chip and the lower chip is hydrophobic, and the microporous array sufficiently separates the generated droplets physically, thereby avoiding cross-contamination. The present invention can effectively control the size and shape of the generated droplet.

Abstract: This invention discloses a self-centering and self-mixing semi-flexible coaxial nozzle capabilities, and its manufacturing method. The invention comprises an upper cover, a semi-flexible inner needle, a three-way casing, a three-way convex platform and an outer needle, wherein the bottom of the upper cover is screwed on the three-way casing; the central part of the three-way casing is provided with the semi-flexible inner needle and the outer needle is screwed on the bottom of the three-way casing; the semi-flexible inner needle extends to the inside of the outer needle through the head of the three-way casing; and two three-way convex platform are screwed on the left and right side surfaces of the three-way casing. The invention has the following beneficial effects: the semi-flexible inner needle self-centers via fluid dynamics to correct coaxiality errors; the three-way mixer enables passive material blending; and a sealed assembly (fixed plate, fixture block) ensures leak-proof rigidity.

Abstract: The present invention provides a method for generating a droplet array on a microfluidic chip, comprising the following steps: assembling an upper chip and a lower chip to an initial position, a fluid channel of the upper chip partially or completely covering a microporous array of the lower chip; injecting a solution into a chip, the solution being partially or fully filled in the microporous array of the lower chip; and relatively moving the upper chip and the lower chip to a liquid dividing position, the fluid channel of the upper chip and the microporous array of the lower chip being overlapped no longer, and the solution being dispersed into the microporous array to form a droplet array. A contact surface between the upper chip and the lower chip is hydrophobic, and the microporous array sufficiently separates the generated droplets physically, thereby avoiding cross-contamination. The present invention can effectively control the size and shape of the generated droplet.