Abstract: A multi-frequency ultrasound coupled multi-nozzle food additive manufacturing device and method are provided. A multi-frequency ultrasound coupling printing system is set up on a multi-nozzle additive manufacturing device, which improves the rheological properties of high-viscosity and large-particle food ink by generating a cavitation effect through the high-frequency vibration of ultrasound and improves the printability of food ink while retaining the nutrients and texture of the food ink. The application of the multi-frequency ultrasound coupled multi-nozzle device to food additive manufacturing enables it to achieve a smaller nozzle diameter and higher printing speed than conventional additive manufacturing systems, which improves the printability of the food ink while realizing large-scale production.

Abstract: An insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof are provided. Through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby.

Abstract: A Bacillus velezensis (B. velezensis) strain bred through ultrasound-assisted adaptive evolution and use thereof are provided. In the present disclosure, a B. velezensis strain (B. velezensis CICC 23571) is used as a starting strain, and an ultrasound-assisted adaptive evolution process is performed, where a proportion of organic nitrogen in a medium is continuously reduced and proportions of inorganic nitrogen and a sugar source are increased to obtain a B. velezensis mutant strain with a high polypeptide yield. Different metabolic pathways in the mutant strain are enhanced, such that the mutant strain can efficiently convert inorganic nitrogen into organic nitrogen or reduce the deaminization of organic nitrogen, and finally a yield of polypeptides is greatly increased.

Abstract: An ultrasonic preparation method of protein peptide-polysaccharide nanoparticles loaded with bioactive components is conducted as follows: Chitosan solution containing 1% glacial acetic acid is added gently to the casein phosphopeptide solution containing quercetin stock solution in an equal volume under constant magnetic stirring; pH is adjusted accordingly before subjecting the mixture solution to ultrasonic treatment; after ultrasonication, the quercetin-loaded casein phosphopeptide-chitosan nanoparticles are obtained by freeze-drying. In the process of using the electrostatic interaction between the casein phosphopeptide and the chitosan to embed the quercetin, the present disclosure employed the multi-mode ultrasonic processing technology to facilitate the cross-linking of the polypeptide and the polysaccharide through the physical force of ultrasound. This enabled the complex encapsulation to incorporate additional bioactive components.

Abstract: An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and ?-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and ?-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.

Abstract: An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and ?-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and ?-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.

Abstract: An ultrasound-assisted simulated digestion method of a milk protein active peptide and an application thereof in health foods, pertaining to the technical field of intensive processing of dairy products and preparation of health foods. The method firstly employs ultrasonic pretreatment of casein and ?-lactoglobulin, followed by enzymatic hydrolysis with a protease to prepare casein and ?-lactoglobulin polypeptide, and traces the activity of the polypeptide by simulating gastrointestinal digestion, and then simulates absorption by intestinal epithelial cells with Caco-2 cells, to characterize a highly active milk protein polypeptide digested by the gastrointestinal tract and absorbed by the Caco-2 cells simulating absorption by the inner wall of the small intestine. The method has identified five such highly active milk protein polypeptides.

Abstract: The invention is about methods for preparing functional polypeptide by multi-mode ultrasound enhancement on enzymolysis, and it relates to the field of functional peptide production technologies. The objective of the invention is to improve the degree of hydrolysis of protein, increase the anti-hypertensive activity of protein hydrolysate, shorten the enzymolysis time and reduce the energy consumption. To achieve this, some methods are developed, such as the method for preparing the anti-hypertensive peptide from wheat gluten by sequential ultrasound enhanced enzymatic hydrolysis, preparation of anti-hypertensive peptide from corn gluten by ultrasonic reverberation field, and the technology for preparing functional polypeptide by countercurrent mode dual frequency ultrasound pretreatment of prion protein. The invention of solid-liquid scanning ultrasonic pretreatment of corn gluten meal suspension leads to an improved efficiency of enzymatic hydrolysis.