Abstract: The present disclosure provides a method and apparatus for training an item copy-writing generation network. The method includes: obtaining item description information of each item in an item set; carrying out data preprocessing on an item description information set corresponding to the item set, to obtain a processed item description information set; training an initial first item copy-writing generation network, to obtain a trained first item copy-writing generation network; and training an initial second item copy-writing generation network through a knowledge distillation method, to obtain a trained second item copy-writing generation network.

Abstract: Provided is a method of inserting a polynucleotide sequence into a genome of a cell. The method comprises: generating a double-strand break at a target site of the genome; and introducing into the cell a virus. The virus comprises a nucleic acid comprising the polynucleotide sequence to be inserted or the complementary sequence thereof. The nucleic acid does not comprise a homologous arm or comprises very short (5˜25 bp) homologous arms corresponding to the target site. Also provided herein is a composition for inserting a polynucleotide sequence into a genome of a cell. The composition comprises a site-specific nuclease capable of generating a DNA double-strand break at a target site of the genome and a virus comprising a nucleic acid comprising the polynucleotide sequence or the complementary sequence thereof.

Abstract: Provided is a method of inserting a polynucleotide sequence into a genome of a cell. The method comprises: generating a double-strand break at a target site of the genome; and introducing into the cell a virus. The virus comprises a nucleic acid comprising the polynucleotide sequence to be inserted or the complementary sequence thereof. The nucleic acid does not comprise a homologous arm or comprises very short (5˜25 bp) homologous arms corresponding to the target site. Also provided herein is a composition for inserting a polynucleotide sequence into a genome of a cell. The composition comprises a site-specific nuclease capable of generating a DNA double-strand break at a target site of the genome and a virus comprising a nucleic acid comprising the polynucleotide sequence or the complementary sequence thereof.

Abstract: Methods and compositions are provided for modulating pancreatic islet ? cell proliferation. Aspects of the methods include promoting ? cell proliferation by providing agents that promote PDGFR signaling, or inhibiting ? cell proliferation by providing agents that inhibit PDGFR signaling. These methods find a number of uses, including, for example, in the treatment of diabetes and human islet diseases. Also provided are reagents and kits thereof that find use in practicing the subject methods.