Abstract: Provided is an anisotropic hydrogel and its preparation method and use. In the preparation method of the anisotropic hydrogel, transglutaminase is added to introduce enzymatic chemical crosslinking, which, in combination with physical crosslinking, enables the hydrogel to be stretched to form anisotropy and stabilizes the resulting anisotropy. The surface of the anisotropic hydrogel exhibits an ordered and oriented structure. When applied to in vitro cell culture, it enables the cells to grow in a uniform direction. When the anisotropic hydrogel is used for preparing cultured meat, the resulting cultured meat presents a fibrous texture close to that of real meat. The preparation method of the anisotropic hydrogel is simple to operate. In the fibrous material dispersion, the fibrous material includes cellulose or protein isolate amyloid fibrils, and these raw materials are non-toxic and harmless, making them suitable for use in the food field.

Abstract: The present disclosure relates to methods aimed towards non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular region. More particularly, the present disclosure relates to methods of using photoselection to achieve non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular regions, via the use of light-activated probes.

Abstract: The present disclosure relates to methods aimed towards non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular region. More particularly, the present disclosure relates to methods of using photoselection to achieve non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular regions, via the use of light-activated probes.

Abstract: The present disclosure relates to methods aimed towards non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular region. More particularly, the present disclosure relates to methods of using photoselection to achieve non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular regions, via the use of light-activated probes.

Abstract: The present disclosure provides compositions and methods for performance of targeted mutagenesis in higher eukaryotic cells, e.g., mammalian cells, across large stretches of targeted sequence. Compositions and methods that rely upon combination of a bacteriophage polymerase with a nucleic acid-editing deaminase to achieve robust mutagenesis of targeted regions of nucleic acid sequence under control of a phage promoter are specifically provided.

Abstract: A method and a system allow presentation of web pages to an internet appliance (e.g., a hand-held computer, a mobile telephone, or a digital personal assistant) according to user preferences. The user preferences are captured by a management server, which provides a web page customization service in conjunction with a document manager, which parses the web pages to identify information units. The customized web pages are stored in a database using a standardized hypertext document representation device, such as XML. The customized web pages are accessible from a portal adapted for accessing by the internet appliance. In one implementation, the user is also offered pre-configured resources for frequently used services when accessing the portal using the internet device.