Abstract: A genetically engineered bacterial strain that produces N-acetylglucosamine, as well as a method of construction and use thereof. The genetically engineered bacterial strain can ferment N-acetylglucosamine under a condition of 40-50° C. Through knocking out genes for glucosamine 6-phosphate deaminase, N-acetylglucosamine-6-phosphate deacetylase and the N-acetylglucosamine transporter protein from a parental bacterium, an N-acetylglucosamine catabolism pathway is blocked. Moreover, overexpression genes for glucosamine 6-phosphate synthase and glucosamine 6-phosphate acetylase are introduced, enabling extra cellular accumulation of N-acetylglucosamine and high-temperature fermentation of N-acetylglucosamine at a temperature higher than 40° C.

Abstract: A composite membrane, a biosensor, and preparation methods thereof are disclosed. In the preparation process, a moldable and plastic material is used as a carrier; after a dispersion liquid of a nanocarbon material wets the carrier, by means of multiple stretching and folding processes, the nanocarbon material is uniformly distributed and arranged regularly inside the entire plastic material in a stretching strain direction. The prepared composite membrane is elastic, attachable, and cost-efficient, and may detect strains as high as 530%. The composite membrane further has high sensitivity and high durability, and may be effectively applied to a biosensor for monitoring motion and humidity.

Abstract: A composite membrane, a biosensor, and preparation methods thereof are disclosed. In the preparation process, a moldable and plastic material is used as a carrier; after a dispersion liquid of a nanocarbon material wets the carrier, by means of multiple stretching and folding processes, the nanocarbon material is uniformly distributed and arranged regularly inside the entire plastic material in a stretching strain direction. The prepared composite membrane is elastic, attachable, and cost-efficient, and may detect strains as high as 530%. The composite membrane further has high sensitivity and high durability, and may be effectively applied to a biosensor for monitoring motion and humidity.