Abstract: For molecular genetic breeding of crops, molecular markers for identifying an allele at a rice-blast-resistant Pik locus of rice and use thereof are provided. The Pik gene is re-sequenced, and through sequence polymorphism analysis, identity codes, 3 SNPs, composed of nucleotide types at reverse sites 806, 901 and 4210 of the Pik-1 start codon have been found, which effectively identify Pik, Pike, Pil, Pikm, and Piks. The molecular markers quickly screen the allele type at the Pik locus, obtain an accurate result just by PCR (Polymerase Chain Reaction) and electrophoresis detection, and has high throughput and low cost.

Abstract: Primer groups for detecting a hybrid rice backbone parent and application thereof. The primer groups according to the invention can distinguish any one of 29 backbone parent materials simply by judging based on a band pattern, without sequencing. Meanwhile, primers according to the invention have high polymorphism and can be applied to the purity detection of hybrid rice seeds for production, where only DNA is extracted from a sample indoor and then detected by using a molecular marker. Therefore, time, labor and cost are saved.