Patents by Inventor Haizhi Huang

Haizhi Huang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD FOR BUILDING CO-CULTURE MODEL FOR CACO-2/RAW-264.7 CELLS INDUCED BY LIPOPOLYSACCHARIDES AND APPLICATION OF CO-CULTURE MODEL
    Publication number: 20240027428
    Abstract: The present invention discloses a method for building a co-culture model for Caco-2/RAW-264.7 cells induced by lipopolysaccharides and an application of the co-culture model. The method includes: 1) culturing Caco-2 cells and RAW-264.7 cells; 2) inoculating the 5 Caco-2 cells onto an AP side of an upper chamber of a Transwell plate, and incubating; 3) inoculating the RAW-264.7 cells onto a 24-well plate, and incubating; 4) transferring the upper chamber of the Transwell plate into the 24-well plate; 5) dissolving lipopolysaccharides in PBS to prepare a stock solution, filtering, and diluting for later use; and 6) adding an LPS-10% DMEM medium on a BL side, incubating, and forming an intestinal inflammation model.
    Type: Application
    Filed: March 13, 2023
    Publication date: January 25, 2024
    Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY
    Inventors: Rongfa Guan, Mengyu Yang, Hao Zhong, Xiaofeng Liu, Donghong Liu, Guozhou Cao, Yong Cheng, Haitao Shen, Jun Huang, Kai Yang, Yanbin Wang, Haizhi Huang