Abstract: Provided is a method for treating ammoniacal nitrogen in wastewater performed via biologically oxidizing ammoniacal nitrogen, and producing nitrite nitrogen and nitrate nitrogen at a desired rate. In the method, ammoniacal nitrogen is oxidized by bacterial sludges to produce at least either of nitrite nitrogen and nitrate nitrogen. Specifically, the method includes an inactivating treatment step of treating the bacterial sludges with an inactivating operation via sterilizing bacteria or causing bacteriostasis, and a nitrifying treatment step of oxidizing the ammoniacal nitrogen by the bacterial sludges thus treated in the inactivating operation. Production amounts of the nitrite nitrogen and the nitrate nitrogen are controlled by adjusting biomass of the bacterial sludges to be treated in the inactivating operation, and/or a time interval between the inactivating operations each repeatedly performed in combination with the nitrifying treatment step.

Abstract: Provided is a method for treating ammoniacal nitrogen in wastewater performed via biologically oxidizing ammoniacal nitrogen, and producing nitrite nitrogen and nitrate nitrogen at a desired rate. In the method, ammoniacal nitrogen is oxidized by bacterial sludges to produce at least either of nitrite nitrogen and nitrate nitrogen. Specifically, the method includes an inactivating treatment step of treating the bacterial sludges with an inactivating operation via sterilizing bacteria or causing bacteriostasis, and a nitrifying treatment step of oxidizing the ammoniacal nitrogen by the bacterial sludges thus treated in the inactivating operation. Production amounts of the nitrite nitrogen and the nitrate nitrogen are controlled by adjusting biomass of the bacterial sludges to be treated in the inactivating operation, and/or a time interval between the inactivating operations each repeatedly performed in combination with the nitrifying treatment step.

Abstract: A method for producing a nitrite type nitrification-reactive sludge includes the step of subjecting an activated sludge to alkali treatment so that the pH of the activated sludge containing at least the ammonium oxidizing bacteria and nitrite oxidizing bacteria can be 10 or higher, in order to accumulate ammonium oxidizing bacteria with higher priority in the activated sludge.

Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.

Abstract: The hydrogen-producing method of the an aspect of the present invention is a method for producing hydrogen in which hydrogen is produced from an organic matter using a microorganism, characterized by using pellets on which hydrogen-producing acid-resistant bacteria are entrapped and immobilized, producing hydrogen by bringing the pellets into contact and react with the organic matter in an environment of a pH of 4 to 6. The inventors of the present invention have obtained a finding that a hydrogen-producing bacteria are entrapped and immobilized, so that the optimum pH of the hydrogen-producing bacterium shifts to low range. The present invention is made based on the above finding and uses pellets on which the hydrogen-producing bacteria are entrapped and immobilized, so that the hydrogen-producing bacterium is activated at a low pH range of 4 to 6 to produce hydrogen. Therefore, the effects of contaminated bacteria that consume hydrogen are less, and the yield of hydrogen can be improved.

Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.

Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.

Abstract: A method for producing a nitrite type nitrification-reactive sludge includes the step of subjecting an activated sludge to alkali treatment so that the pH of the activated sludge containing at least the ammonium oxidizing bacteria and nitrite oxidizing bacteria can be 10 or higher, in order to accumulate ammonium oxidizing bacteria with higher priority in the activated sludge.

Abstract: The hydrogen-producing method of the an aspect of the present invention is a method for producing hydrogen in which hydrogen is produced from an organic matter using a microorganism, characterized by using pellets on which hydrogen-producing acid-resistant bacteria are entrapped and immobilized, producing hydrogen by bringing the pellets into contact and react with the organic matter in an environment of a pH of 4 to 6. The inventors of the present invention have obtained a finding that a hydrogen-producing bacteria are entrapped and immobilized, so that the optimum pH of the hydrogen-producing bacterium shifts to low range. The present invention is made based on the above finding and uses pellets on which the hydrogen-producing bacteria are entrapped and immobilized, so that the hydrogen-producing bacterium is activated at a low pH range of 4 to 6 to produce hydrogen. Therefore, the effects of contaminated bacteria that consume hydrogen are less, and the yield of hydrogen can be improved.

Abstract: Provided is a structure designed for adsorption, which is suitable for removing dioxin and dioxin-like substances from leachate and ground water from polluted soils or garbage, washing effluent from garbage incinerators and so on that contain DNA intercalators, particularly, dioxin and dioxin-like substances. The structure designed for adsorption is a structure designed for adsorption having an adsorbing layer containing, as a constituent, a DNA complex containing a DNA-binding protein, DNA including double strand DNA and a carrier, which can selectively remove DNA intercalators with high efficiency from water, gas and so on containing them.

Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.

Abstract: A microorganism separation device includes: sample solution supply means (30) for supplying a sample solution (40) stored within a sample solution reservoir (32) to a first flow path (12); a microorganism sensor (22) that is capable of detecting a monadelphous microorganism in the sample solution (40) that passes through the first flow path (12); a controller (24) that stops to supply the sample solution (40) to the first flow path (12) and discharges the detected microorganism together with the sample solution (40) from a termination side of the first flow path (12) on the basis of a detection result of the microorganism by the microorganism sensor (22); and an acceptor (52) that receives a droplet (28) of the sample solution (40) that is discharged from the termination side of the first flow path (12).

Abstract: A microorganism separation system comprising a sample solution container 34 containing microorganisms, a separator 1, and a receiver 47, designed to separate microorganisms from the sample solution; further comprising a microorganism detection sensor and a plate 49 which has a plurality of receivers 47 connected to each other and an identification indicator, wherein when the microorganism detection sensor judges that a microorganism has passed, supply of the sample solution is stopped, the detected microorganism is discharged together with the sample solution, and then the solution starts to be injected into the receiver; and, the number of times microorganisms are detected during the time period from the start of the injection to the end is recognized as separation quantity; and then as separation information, the separation quantity, number for a receiver 47 into which a microorganism was injected for each identification indicator, signal waveform sent from the microorganism detection sensor at the separ

Abstract: Provided is a structure designed for adsorption, which is suitable for removing dioxin and dioxin-like substances from leachate and ground water from polluted soils or garbage, washing effluent from garbage incinerators and so on that contain DNA intercalators, particularly, dioxin and dioxin-like substances. The structure designed for adsorption is a structure designed for adsorption having an adsorbing layer containing, as a constituent, a DNA complex containing a DNA-binding protein, DNA including double strand DNA and a carrier, which can selectively remove DNA intercalators with high efficiency from water, gas and so on containing them.