Patents by Inventor Hajime Sakai

Hajime Sakai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Organelle genome modification using polynucleotide guided endonuclease
    Patent number: 11920140
    Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
    Type: Grant
    Filed: July 18, 2022
    Date of Patent: March 5, 2024
    Assignee: NAPIGEN, INC.
    Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, Jr., Ganesh Kishore, Narendra Singh Yadav
  • PHOSPHITE DEHYDROGENASE AS A SELECTABLE MARKER FOR MITOCHONDRIAL TRANSFORMATION
    Publication number: 20230175003
    Abstract: The present disclosure relates to genetically modified cells containing mitochondria that have been transformed with a polynucleotide encoding a phosphite dehydrogenase enzyme, such that the cells can utilize phosphite as a phosphorus source.
    Type: Application
    Filed: December 6, 2022
    Publication date: June 8, 2023
    Inventors: Narendra Yadav, Hajime Sakai, Dilbag Multani, Cheryl Caster, Emil Orozco, Ganesh Kishore
  • Nucleotide sequences encoding Fasciated EAR3 (FEA3) and methods of use thereof
    Patent number: 11643665
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Grant
    Filed: February 6, 2020
    Date of Patent: May 9, 2023
    Assignees: CORTEVA AGRISCIENCE LLC, COLD SPRING HARBOR LABORATORY
    Inventors: Stephen M Allen, David Peter Jackson, Byoung Il Je, Mai Komatsu, Young Koung Lee, Hajime Sakai
  • ORGANELLE GENOME MODIFICATION USING POLYNUCLEOTIDE GUIDED ENDONUCLEASE
    Publication number: 20230123175
    Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
    Type: Application
    Filed: July 18, 2022
    Publication date: April 20, 2023
    Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, JR., Ganesh Kishore, Narendra Singh Yadav
  • ORGANELLE GENOME MODIFICATION
    Publication number: 20220372523
    Abstract: Provided herein are methods and compositions for modifying cells. Provided herein are methods and compositions for modifying an organism of a cell. Provided herein are methods and compositions for introducing polynucleotides and/or polypeptides into a nucleus of a cell.
    Type: Application
    Filed: December 23, 2021
    Publication date: November 24, 2022
    Inventors: Emil Meyer OROZCO, JR., Narendra Singh YADAV, Hajime SAKAI
  • ORGANELLE GENOME MODIFICATION USING POLYNUCLEOTIDE GUIDED ENDONUCLEASE
    Publication number: 20210054404
    Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
    Type: Application
    Filed: August 22, 2018
    Publication date: February 25, 2021
    Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, Jr., Roger Wyse, Ganesh Kishore, Jay Keasling, Narendra Singh Yadav
  • NUCLEOTIDE SEQUENCES ENCODING FASCIATED EAR3 (FEA3) AND METHODS OF USE THEREOF
    Publication number: 20200157557
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Application
    Filed: February 6, 2020
    Publication date: May 21, 2020
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, COLD SPRING HARBOR LABORATORY
    Inventors: STEPHEN M ALLEN, DAVID PETER JACKSON, BYOUNG IL JE, MAI KOMATSU, YOUNG KOUNG LEE, HAJIME SAKAI
  • Nucleotide sequences encoding fasciated EAR3 (FEA3) and methods of use thereof
    Patent number: 10584349
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: March 10, 2020
    Assignee: E I DU PONT DE NUMOURS AND COMPANY COLD SPRING HARBOR LABORATORY
    Inventors: Stephen M. Allen, David Peter Jackson, Byoung Il Je, Mai Komatsu, Young Koung Lee, Hajime Sakai
  • ORGANELLE GENOME MODIFICATION USING POLYNUCLEOTIDE GUIDED ENDONUCLEASE
    Publication number: 20190136249
    Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
    Type: Application
    Filed: August 22, 2018
    Publication date: May 9, 2019
    Inventors: Hajime SAKAI, Byung-Chun YOO, Emil Meyer OROZCO, JR., Roger WYSE, Ganesh KISHORE, Jay KEASLING, Narendra Singh YADAV
  • Nucleotide sequences encoding fasciated EAR4 (FEA4) and methods of use thereof
    Patent number: 9850495
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of Fea4 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: December 26, 2017
    Assignees: E. I. du Pont de Nemours And Company, Cold Spring Harbor Laboratory, Iowa State University Research Foundation, Inc.
    Inventors: Stephen M Allen, David Peter Jackson, Mai Komatsu, Michael Pautler, Hajime Sakai, Erik Vollbrecht, Rebecca Weeks
  • NUCLEOTIDE SEQUENCES ENCODING FASCIATED EAR3 (FEA3) AND METHODS OF USE THEREOF
    Publication number: 20170275642
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Application
    Filed: June 2, 2017
    Publication date: September 28, 2017
    Applicants: E I Du Pont De Nemours and Company, Cold Spring Harbor Laboratory
    Inventors: Stephen M. Allen, David Peter Jackson, Byoung IL Je, Mai Komatsu, Young Koung Lee, Hajime Sakai
  • DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING DTP6 POLYPEPTIDES
    Publication number: 20170211088
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP6 polypeptide.
    Type: Application
    Filed: April 7, 2017
    Publication date: July 27, 2017
    Inventors: STEPHEN M. ALLEN, HONOR RENEE LAFITTE, JASON L. BROTHERS, KRUPA DESHMUKH, XIAO-YI LI, CHENG LU, STANLEY LUCK, JEFFREY MULLEN, HAJIME SAKAI, JAMES J SAYLOR, SCOTT V. TINGEY, ROBERT WAYNE WILLIAMS
  • Nucleotide sequences encoding fasciated EAR3 (FEA3) and methods of use thereof
    Patent number: 9701979
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: July 11, 2017
    Assignee: E I DU PONT DE NEMOURS AND COMPANY COLD SPRING HARBOR LABORATORY
    Inventors: Stephen M Allen, David Peter Jackson, Byoung Il Je, Mai Komatsu, Young Koung Lee, Hajime Sakai
  • Drought tolerant plants and related constructs and methods involving genes encoding DTP6 polypeptides
    Patent number: 9624504
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP6 polypeptide.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: April 18, 2017
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Stephen M Allen, Jason L Brothers, Krupa Deshmukh, Honor Renee Lafitte, Xiao-Yi Li, Cheng Lu, Stanley Luck, Jeffrey Mullen, Hajime Sakai, James J Saylor, Scott V Tingey, Robert Wayne Williams
  • Drought tolerant plants and related constructs and methods involving genes encoding DTP21 polypeptides
    Patent number: 9556450
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.
    Type: Grant
    Filed: December 15, 2014
    Date of Patent: January 31, 2017
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, JAPAN TOBACCO INC, PIONEER HI-BRED INTL INC.
    Inventors: Marc C Albertsen, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
  • Methods for the identification of genes involved in abiotic stress tolerance in plants
    Patent number: 9546377
    Abstract: Methods are described for the identification of genes useful for conferring tolerance in plants to abiotic stress. Transgenic plants and seeds comprising the stress tolerant genes are also described. Methods to monitor the growth rate of the transgenic plants under stressed or non-stressed conditions are also described.
    Type: Grant
    Filed: October 29, 2012
    Date of Patent: January 17, 2017
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Bindu Andreuzza, Milo Aukerman, Jason L. Brothers, Norbert Brugiere, Mai Komatsu, Ratna Kumria, Xiao-Yi Li, Cheng Lu, Amitabh Mohanty, Hajime Sakai, James J. Saylor, Sobhana Sivasankar, Graziana Taranino, Robert Wayne Williams
  • DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING DTP4 POLYPEPTIDES
    Publication number: 20160369295
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring stress tolerance are presented herein, along with compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP4 polypeptide.
    Type: Application
    Filed: December 22, 2014
    Publication date: December 22, 2016
    Applicants: Pioneer Hi-Bred International Inc., E. I. du Pont de Nemours and Company
    Inventors: Stephen M. Allen, Bindu Andreuzza, Norbert Brugiere, Zhenglin Hou, Ratna Kumria, H. Renee Lafitte, Xiao-Yi Li, Cheng Lu, Stanley Luck, Amitabh Mohanty, Jeffery Mullen, Rupa Raja, Hajime Sakai, Scott V. Tingey, Robert W. Williams
  • POLYNUCLEOTIDES AND POLYPEPTIDES INVOLVED IN POST-TRANSCRIPTIONAL GENE SILENCING
    Publication number: 20160355834
    Abstract: This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the the polypeptide involved in post-transcriptional gene silencing.
    Type: Application
    Filed: August 23, 2016
    Publication date: December 8, 2016
    Inventors: Rebecca E. Cahoon, HAJIME SAKAI, J ANTONI RAFALSKI
  • Polynucleotides and polypeptides involved in post-transcriptional gene silencing
    Patent number: 9453236
    Abstract: This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the polypeptide involved in post-transcriptional gene silencing.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: September 27, 2016
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Rebecca E. Cahoon, Hajime Sakai, J. Antoni Rafalski
  • Skin analyzer for skin care
    Patent number: D985129
    Type: Grant
    Filed: January 15, 2021
    Date of Patent: May 2, 2023
    Assignee: The Procter & Gamble Company
    Inventors: Naoki Miyamoto, Koji Yamamoto, Hajime Sakai