Abstract: Provided is a method in which visibility of colonies of microorganisms in a medium is improved and quantitative counting of the number of microorganisms can be simply and accurately performed. The method of counting the number of microorganisms includes a step of adding a analyte to a composition containing (a) a polymer compound that can form non-flowable transparent gel without passing through dissolution by heating and without depending on cooling, (b) guar gum and (c) a nutritional ingredient and mixing the resulting mixture, a step of culturing the microorganisms contained in the analyte, and a step of counting the number of colonies of the microorganisms.

Abstract: To provide a culture medium in which plural kinds of bacteria of genus Clostridium can be selectively detected on the same culture medium and identified for every species. A culture medium for detecting bacteria of genus Clostridium contains (a) cycloserine, (b) polymyxin B, (c) an aminoglycoside-based antibiotic, (d) sodium taurocholate and (e) a phosphatase substrate capable of releasing a colored chromogen compound.

Abstract: Provided is a method for simply and accurately counting the number of microorganisms in an analyte containing carbonate ion or bicarbonate ion in a dialysis fluid or the like. A counting method for the number of microorganisms includes: a step of adding the analyte to a composition for preparing a medium for counting the number of microorganisms, and containing (a) polyacrylic acid and/or a salt thereof, (b) hydroxide of alkaline earth metal and (c) a nutritional ingredient, and mixing the resulting mixture; a step of culturing microorganisms contained in the analyte; and a step of counting the number of colonies of the microorganisms.

Abstract: An object is to provide a culture medium in which Enterococcus can be selectively detected. The culture medium for detecting Enterococcus contains an ionic surfactant and polyvinyl alcohol. The culture medium for detecting Enterococcus may further contain a suppressant on Gram-negative bacteria and a ?-glucosidase substrate from which a colored chromogen compound can be released.

Abstract: A method of measuring simply and accurately a microbial count in a sample. Namely, a method of measuring a microbial count including a step of blending a composition for preparing a culture medium for a microbial count measurement including (a) a polymer able to form a non-flowable transparent gel without a melting step by heating and without cooling, and (b) a nutrient component, with a sample added thereto, a step of incubating microorganisms contained in the sample, and a step of measuring the colony count of the microorganisms.

Abstract: A culture equipment in which culture equipment can be easily produced with high handleability, and the number of the microorganisms in the analyte can be easily counted. The culture equipment of microorganisms has (a) an upper member, (b) a lower member having a recess, and (c) a medium ingredient, and (c) the medium ingredient contains (c1) at least one selected from polyacrylic acid and/or a salt thereof and a derivative thereof, and (c2) a nutritional ingredient. Then, (a) the upper member preferably has a projection having a shape that can be mutually fitted to the recess of (b) the lower member through (c) the medium ingredient.

Abstract: To provide a method in which staining of a culture medium itself can be suppressed even if an enzyme that can decompose an enzyme substrate to be decomposed by an enzyme of microorganisms inherently exists in an analyte, and target microorangisms can be detected as stained colony with high accuracy, and an enzyme substrate culture medium therefor. A culture medium for detecting microorganisms includes a first layer in which a gelling agent and an enzyme substrate are contained, and a second layer in which a gelling agent is contained and the enzyme substrate is not contained to be laminated adjacently to the first layer, wherein the enzyme substrate is a compound from which a dye compound can be released.

Abstract: A solution is the microorganism testing apparatus equipped with a pre-processing unit in which, if a content of microorganism testing on a specific specimen is selected or input, testing conditions of the specimen read from a storage device in which testing information specifying the testing conditions of the specimen is stored are printed on a sheet-form medium to be used for testing of the specimen, and a post-processing unit in which, if an image of the medium is read, the testing conditions printed on the medium and a type of the medium preliminarily labelled on the medium are acquired from the image to output judgment results as to whether or not the testing conditions acquired from the image, and the type of the medium are compatible.

Abstract: A solution is the microorganism testing apparatus equipped with a pre-processing unit in which, if a content of microorganism testing on a specific specimen is selected or input, testing conditions of the specimen read from a storage device in which testing information specifying the testing conditions of the specimen is stored are printed on a sheet-form medium to be used for testing of the specimen, and a post-processing unit in which, if an image of the medium is read, the testing conditions printed on the medium and a type of the medium preliminarily labelled on the medium are acquired from the image to output judgment results as to whether or not the testing conditions acquired from the image, and the type of the medium are compatible.

Abstract: A selective isolation medium that is not influenced by existence of ESBL-producing bacteria and can clearly detect Campylobacter bacteria. Cefoperazone and Cefoxitin are incorporated into the culture medium for detecting Campylobacter bacteria.

Abstract: A sheet-form microorganism culturing material including a water-soluble polymer compound layer and a porous matrix layer in which both high moisture retention capability and colony color formation capability are satisfied. The microorganism culturing material includes a first layer containing a porous material, and a second layer adjacent to the first layer and containing a gelling agent, wherein the microorganism culturing material contains polyvinyl alcohol and methylcellulose or guar gum as the gelling agent.