Patents by Inventor Hal S. Padgett
Hal S. Padgett has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7273739Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: May 3, 2006Date of Patent: September 25, 2007Inventors: Hal S. Padgett, Andrew A. Vaewhongs
-
Patent number: 7235386Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: July 25, 2002Date of Patent: June 26, 2007Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
-
Patent number: 7217514Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: July 25, 2002Date of Patent: May 15, 2007Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
-
Patent number: 7078211Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: August 1, 2002Date of Patent: July 18, 2006Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, Andrew A. Vaewhongs
-
Patent number: 7056740Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.Type: GrantFiled: January 31, 2003Date of Patent: June 6, 2006Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith, John A. Lindbo, Wayne P. Fitzmaurice
-
Publication number: 20040180352Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: August 8, 2003Publication date: September 16, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
-
Publication number: 20040142433Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.Type: ApplicationFiled: October 10, 2003Publication date: July 22, 2004Inventors: Hal S. Padgett, Wayne P. Fitzmaurice, John A. Lindbo, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith
-
Publication number: 20040110130Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.Type: ApplicationFiled: October 25, 2002Publication date: June 10, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
-
Publication number: 20030219878Abstract: Restriction Independent Cloning Events (RICE) are made by generating 5′ overhangs (sticky ends). The polynucleotides to be joined are reacted with a DNA polymerase, having 3′ to 5′ exonuclease activity and 5′ to 3′ polymerizing activity, less than all of the dNTPs, a kinase (optional) and a ligase. The complementary 5′ overhangs anneal and ligate.Type: ApplicationFiled: November 1, 2002Publication date: November 27, 2003Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: John A. Lindbo, Hal S. Padgett
-
Publication number: 20030186261Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: July 25, 2002Publication date: October 2, 2003Applicant: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
-
Publication number: 20030166169Abstract: The present invention relates to a method for constructing viral nucleic acids in a cell-free manner. In essence, the cell-free method entails the immobilization of a fragment of a double-stranded DNA sequence on a solid support and the assembly of the remaining fragments of the double-stranded DNA sequence onto the immobilized fragment. If the viral nucleic acid is derived from an RNA virus, the instant method further comprises the step of in vitro transcription of the assembled double-stranded DNA sequence to yield an RNA viral nucleic acid.Type: ApplicationFiled: July 15, 2002Publication date: September 4, 2003Inventors: Hal S. Padgett, John A. Lindbo
-
Publication number: 20030157495Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: March 14, 2002Publication date: August 21, 2003Inventors: Hal S. Padgett, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith
-
Publication number: 20030157682Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.Type: ApplicationFiled: January 31, 2003Publication date: August 21, 2003Inventors: Hal S. Padgett, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith, John A. Lindbo, Wayne P. Fitzmaurice
-
Publication number: 20030148315Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: August 1, 2002Publication date: August 7, 2003Inventors: Hal S. Padgett, Andrew A. Vaewhongs
-
Publication number: 20030036641Abstract: The invention provides methods of forcing recombination between polynucleotides. The methods can include the steps of, (a) generating a single strand of a first polynucleotide; (b) generating a single strand of a second polynucleotide, wherein the second polynucleotide is partially complementary to the first polynucleotide; (c) fragmenting the single strand of the first polynucleotide to generate single stranded first polynucleotide fragments; (d) fragmenting the single strand of the second polynucleotide to generate single stranded second polynucleotide fragments; (e) annealing the single stranded first polynucleotide fragments with the single stranded second polynucleotide fragments; and (f) extending the annealed polynucleotide fragments.Type: ApplicationFiled: January 31, 2001Publication date: February 20, 2003Inventors: Hal S. Padgett, Wayne P. Fitzmaurice, John A. Lindbo
-
Publication number: 20030027173Abstract: The present invention provides methods for rapidly determining the function of nucleic acid sequences by transfecting the same into a host organism to effect expression. Phenotypic and biochemical changes produced thereby are then analyzed to ascertain the function of the nucleic acids which have been transfected into the host organism. The invention also provides methods for silencing endogenous genes by transfecting hosts with nucleic acid sequences to effect expression of the same. The present invention also provides methods for selecting desired functions of RNAs and proteins by the use of virus vectors to express libraries of nucleic acid sequence variants. Moreover, the present invention provides methods for inhibiting an endogenous protease of a plant host.Type: ApplicationFiled: February 5, 2002Publication date: February 6, 2003Inventors: Guy Della-Cioppa, Robert L. Erwin, Wayne P. Fitzmaurice, Kathleen Hanley, Monto H. Kumagai, John A. Lindbo, David R. McGee, Hal S. Padgett, Gregory P. Pogue
-
Publication number: 20020177160Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: July 25, 2002Publication date: November 28, 2002Applicant: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
-
Patent number: 6468745Abstract: The present invention relates to a method for using viral vectors to bear populations of sequence variants and using plant hosts to select the sequences that exhibit the desired traits.Type: GrantFiled: July 21, 1999Date of Patent: October 22, 2002Assignee: Large Scale Biology CorporationInventors: Wayne P. Fitzmaurice, John A. Lindbo, Hal S. Padgett, Gregory P. Pogue
-
Publication number: 20020146732Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: February 1, 2002Publication date: October 10, 2002Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice