Abstract: The present invention relates to a method of detecting n target nucleic acids in a sample, and more particularly, to a method of detecting n target nucleic acids in a sample in a real-time manner in one reaction vessel by being configured such that signals from one or two target nucleic acids are detected at each of n (n is an integer of 3 or more) detection temperatures despite the use of a single fluorescent label.

Abstract: Proposed is a method for obtaining a prediction result of a nucleic acid amplification reaction affected by an nth-order structure, which is performed by a computing device. The method may include accessing a prediction model learned using a plurality of training data Each training data may include a first analysis data for an nth-order structure in a nucleic acid sequence and an amplification reaction result for the nucleic acid sequence, where n is an integer not less than 2. The method may also include obtaining an input data comprising a second analysis data for an nth-order structure in a target nucleic acid sequence. The method may further include providing the input data to the prediction model, and obtaining a prediction result of an amplification reaction for the target nucleic acid sequence from the prediction model.

Abstract: The present invention relates to a method for predicting the melting temperature (Tm) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tm of an oligonucleotide in various reaction environments using the equations for Tm calculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.

Abstract: A method for obtaining an approximate signal for each of a plurality of target analytes performed by a computer device includes: obtaining, from a signal generation reaction for a first target analyte and a second target analyte in a sample, a first data set measured at a first detection temperature and a second data set measured at a second detection temperature; and by using the first and the second data set, performing a joint-estimation on (a) a value of a parameter of at least one among an approximate function for approximating signal values dependent on the presence of the first target analyte and an approximate function for approximating signal values dependent on the presence of the second target analyte; and (b) a value of a magnitude parameter indicating a relationship of signal values dependent on the presence of the first target analyte between the first and the second detection temperature.

Abstract: The present invention relates to a method for predicting the melting temperature (Tm) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tm of an oligonucleotide in various reaction environments using the equations for Tm calculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.

Abstract: The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.

Abstract: The present disclosure pertains to a method for detecting multiple target nucleic acids by a single type of label alone in a single reaction vessel by using multiple detection temperatures, which is characterized by providing a signal change dependent on the presence of a corresponding target nucleic acid at a corresponding detection temperature of each of the target nucleic acids. The conventional techniques using a single type of label is subjected to melting analysis after target amplification so as to detect multiple target nucleic acids. In contrast, the present method does not require melting curve analysis after target amplification, even using a single type of label, and thus can remarkably reduce the analysis time.

Abstract: The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.

Abstract: The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reflective value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal values of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instruments) regardless of manufacture. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.

Abstract: The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reference value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.

Abstract: The present invention relates to extraction of a signal for a target nucleic acid sequence from signals for two target nucleic acid sequences in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention using an amended reference value as well as an initial reference value can lead to increasing the detection accuracy in methods for detecting target nucleic acid sequences using different detection temperatures and reference values.

Abstract: The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

Abstract: The present invention relates to the detection of a target nucleic acid sequence by a PTOCE-E (PTO Cleavage and Extension-dependent Extension) assay. The PTOCE-E assay of the present invention can reduce the non-target signal and increase the target signal as compared with the conventional PTOCE and PCE-SH methods, thereby enabling more accurate detection of the target nucleic acid sequence.

Abstract: The present invention relates to the detection of a target analyte in a sample using an S-shaped function for a slope data set. The method of the present invention comprises obtaining a first data set representing a growth curve by an amplification reaction for the target analyte; wherein said first data set includes a plurality of data points, each having a cycle number and a signal value at the cycle number; calculating a slope value at each cycle number for the first data set to obtain a second data set; wherein said second data set includes a plurality of data points, each having a cycle number and a slope value at the cycle number; calculating an S-shaped function that approximates a selected portion of the second data set; and using the S-shaped function to detect the target analyte in a sample.

Abstract: The present invention relates to a method for reducing a noise level of a data set for a target analyte in a sample. The present invention can reduce a noise level of a data set to a proper level conveniently by applying a noise-reduction ratio to the data set thereby the possibility of false positive may be reduced effectively. According to the present invention, the calibrated data set is obtained by using the noise-reduction ratio, so that the noise level of a data set is reduced without change of signal ratio between the data point in the data set.

Abstract: The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

Abstract: The present invention relates to a method for predicting the melting temperature (Tm) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tm of an oligonucleotide in various reaction environments using the equations for Tm calculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.

Abstract: The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.

Abstract: The present invention relates to the detection of a target nucleic acid sequence by a PTOCE-E (PTO Cleavage and Extension-dependent Extension) assay. The PTOCE-E assay of the present invention can reduce the non-target signal and increase the target signal as compared with the conventional PTOCE and PCE-SH methods, thereby enabling more accurate detection of the target nucleic acid sequence.

Abstract: The present invention relates to a method for calibrating a data set of a target analyte in a sample using an analyte-in-susceptible signal value, wherein the analyte-insusceptible signal value is provided by a background-representing signal value of the data set or by a total signal change value of a standard data set. The present method is very convenient and effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.