Abstract: Disclosed herein are methods, compositions and systems for the interrogation of macromolecules, more particularly for preparation of isolated single macromolecules for subsequent processing of specific regions of interest within said macromolecule based on an analysis of the molecule's physical map. The disclosure is further related to the controlled segmentation of long nucleic acid parent molecules into smaller child molecules in a targeted manner such that further processing on said children may be performed with the knowledge of their origin within the parent, in a controlled environment enabled by purposefully designed microfluidic devices. Also disclosed is binding of regional specific barcodes along the length of a long nucleic acid molecule such that upon cleavage of said molecule into child molecules, the regional origin of the children can be tracked, in a controlled environment enabled by purposefully designed microfluidic devices.

Abstract: An all-terrain vehicle includes a frame, wheels, a vehicle cover, a power supply system, a saddle assembly, a brake system, a drive assembly and a suspension system. The all-terrain vehicle is sized for children to ride, with numerous disclosed modifications in accordance with its size and riders. For instance, the saddle assembly includes a locking hook accessible from the rear of the vehicle, and in the locked state holds the main power supply down into its compartment. The brake system uses a pawl for two handed setting of the parking brake. The instrument assembly allows adjustment of the display angle of the display screen. The charger cover uses a damping block to cause the cover to hover at a selected position. The all-terrain vehicle can meet the use needs and safety requirements of all-terrain vehicles for kids, and can ensure the safety of drivers.

Abstract: Provided are a testing system and a testing method for supercritical carbon dioxide continuous flow corrosion. The testing system includes a gas supply system and a closed-loop testing system loop. The testing system loop includes: a circulation pump providing a power of boosting and flowing for a circulation of liquid carbon dioxide in the testing system loop; a flow meter used to measure a flow rate of the liquid carbon dioxide; a heater used to transform the liquid carbon dioxide into a carbon dioxide gas; a test section used for storing a sample and performing a continuous corrosion test; a regenerator used to exchange heat with the tested carbon dioxide gas; a condenser used to condense the heat-exchanged carbon dioxide gas; and a circulation tank used to provide the liquid carbon dioxide for the testing system loop, recycle the liquid carbon dioxide, and form a closed-loop testing system loop.

Abstract: Provided are a testing system and a testing method for supercritical carbon dioxide continuous flow corrosion. The testing system includes a gas supply system and a closed-loop testing system loop. The testing system loop includes: a circulation pump providing a power of boosting and flowing for a circulation of liquid carbon dioxide in the testing system loop; a flow meter used to measure a flow rate of the liquid carbon dioxide; a heater used to transform the liquid carbon dioxide into a carbon dioxide gas; a test section used for storing a sample and performing a continuous corrosion test; a regenerator-connected used to exchange heat with the tested carbon dioxide gas; a condenser used to condense the heat-exchanged carbon dioxide gas; and a circulation tank, used to provide the liquid carbon dioxide for the testing system loop, recycle the liquid carbon dioxide, and form a closed-loop testing system loop.

Abstract: Genome whole chromosome analysis is a critical gold standard method for clinicians to study molecular lesions at single cell level in diseases and cancers. However, they suffer from low-sample-yielding, labor-intensive procedures that require subjective manual involvement of highly trained professionals. Here we disclose new devices, systems, and methods to automate, standardize, and accelerate the digital cytopathological sample and data generation with improved data quality to enable precision medicine and improve clinical practice. This disclosure describes a family of micro-fluidic devices, and accompanying methods for using said devices, to perform cytogenomic analysis on cells, nucleus, vesicles exosomes and their respective chromosomes, chromatins, nucleic acid polymers, subcellular genomic elements and any other dynamic transitional states between these forms.

Abstract: A pressure washer includes a port for a liquid source, a high-pressure pump, and a main line conveying liquid from the port to a spray opening by the high-pressure pump. A main line valve is arranged in the main line and is switchable between a closed state, in which liquid flow is stopped, and a fully open state, in which liquid flows through the main line. An operating device includes a valve control element and a pressure control element. The valve control element switches the main line valve between the closed and fully open states, while the pressure control element, operable independently of the valve control element, specifies the pressure of the liquid in the main line between the high-pressure pump and the spray opening, particularly when the main line valve is fully open.

Abstract: An all-terrain vehicle includes a frame, wheels, a vehicle cover, a power supply system a saddle assembly, a brake system, a drive assembly and a suspension system. The all-terrain vehicle is sized for children to ride, with numerous disclosed modifications in accordance with its size and riders. For instance, the saddle assembly includes a locking hook accessible from the rear of the vehicle, and in the locked state holds the main power supply down into its compartment. The brake system uses a pawl for two handed setting of the parking brake. The instrument assembly allows adjustment of the display angle of the display screen. The charger cover uses a damping block to cause the cover to hover at a selected position. The all-terrain vehicle can meet the use needs and safety requirements of all-terrain vehicles for kids, and can ensure the safety of drivers.

Abstract: This application provides a positioning mechanism, a flexible printed circuit, a flexible printed circuit assembly, and an electronic device. The solution includes a positioning mechanism configured to be arranged on a base. The positioning mechanism includes: at least two positioning members, arranged at intervals on the base along a length direction of the base; and a limiting member, arranged between the two positioning members, where each of the positioning members is provided with an clamping structure, two ends of the limiting member respectively extend to the clamping structures corresponding to the two positioning members, the clamping structure is configured to limit movement of the limiting member in a thickness direction of the base, and a flexible printed circuit is arranged between the limiting member and the base.

Abstract: According to some embodiments herein, methods and kits for labeling and analyzing nucleic acids are provided. In some embodiments, sequence-specific labeling is performed on polynucleotide sequences associated with a host genome, and the presence or absence of patterns characteristic of extragenomic sequences are determined.

Abstract: Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples.

Abstract: Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

Abstract: Systems are provided for detecting and quantitating short nucleic acid molecules.

Abstract: Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.

Abstract: A first set of values reported by an electronic device and not reported by another electronic device over a first period of time that is prior to a firmware release to the electronic device is received. The first set of values is associated with a metric. A set of statistical properties associated with the first set of values is determined. A second set of values reported by the electronic device and not reported by another electronic device over a second period of time that is after the firmware release is received. The second set of values is associated with the metric. A set of statistical properties associated with the second set of values is determined. The set of statistical properties associated with the first set of values and the set of statistical properties associated with the second set of values is compared to detect an anomaly.

Abstract: The invention relates to a cosmetic composition comprising a low molecular weight silk fibroin and a cosmetically acceptable carrier; wherein said low molecular weight silk fibroin comprises at least 85 parts by weight peptides of molecular weight less than 2500 Da, where amino acid sequence of at least 50 wt % of said peptides is GAGY, GAGAGAGY, GAGVGAGY or AWSSESDF and where said silk fibroin is produced by a process comprising the steps of: (i) mixing an aqueous solution comprising 0.01 to 20 wt % of a high molecular weight silk fibroin of weight average molecular weight 6 to 100 KDa with ochymotrypsin at temperature of 25 to 45° C.

Abstract: A first set of values reported by an electronic device and not reported by another electronic device over a first period of time that is prior to a firmware release to the electronic device is received. The first set of values is associated with a metric. A set of statistical properties associated with the first set of values is determined. A second set of values reported by the electronic device and not reported by another electronic device over a second period of time that is after the firmware release is received. The second set of values is associated with the metric. A set of statistical properties associated with the second set of values is determined. The set of statistical properties associated with the first set of values and the set of statistical properties associated with the second set of values is compared to detect an anomaly.

Abstract: A first set of values reported by an electronic device and not reported by another electronic device over a first period of time that is prior to a firmware release to the electronic device is received. The first set of values is associated with a metric. A set of statistical properties associated with the first set of values is determined. A second set of values reported by the electronic device and not reported by another electronic device over a second period of time that is after the firmware release is received. The second set of values is associated with the metric. A set of statistical properties associated with the second set of values is determined. The set of statistical properties associated with the first set of values and the set of statistical properties associated with the second set of values is compared to detect an anomaly.

Abstract: Provided are integrated analysis devices having features of macroscale and nanoscale dimensions, and devices that have reduced background signals and that reduce quenching of fluorophores disposed within the devices. Related methods of manufacturing these devices and of using these devices are also provided.

Abstract: Methods of double-stranded nucleic acid sequence determination and assembly that are able to identify insertions, deletions, repeat region sizes and genomic rearrangements, for example, are disclosed herein, which can use relatively large labeled nucleic acid fragments to analyze the structure of even larger genetic regions. In some embodiments these methods involve the use of certain parameters which unexpectedly improve overall method performance. In some embodiments these methods involve sample labeling that does not result in the formation of single-stranded nucleic acid fragment labeling intermediaries.