Abstract: The present invention provides an inactivated encephalomyocarditis virus (EMCV) vaccine produced from swine isolates which protects swine from diseases caused by EMCV. The vaccine is produced from swine isolates and protects against respiratory problems, myocardial and pulmonary inflammation and necrosis, and confers protection against reproductive disorders resulting from some strains of the virus.

Abstract: A radial immunodiffusion enzyme assay method for the simple estimation of immunoglobulins in humans and other animals, Agar test plates are provided including an underlying adherent coating of Staphylococcal Protein A antigen. Blood or blood serum samples from animals to be tested are placed in wells punched in the agar layer and allowed to incubate overnight. The agar gel is then removed. The resulting Protein A antigen layer with bound immunoglobulins from the samples is reacted with enzyme conjugated anti-immunoglobulin or enzyme conjugated Protein A. The reaction is visualized by overlaying the bound conjugate layer with agar containing a color producing enzyme substrate. The diameters of resulting colored zones permit estimation of total immunoglobulins. Methods of preparing Protein A antigen coated test plates are disclosed along with testing kits for carrying out the test procedure in the field.

Abstract: A radial immunodiffusion enzyme assay method for the simple testing of pseudorabies antibodies in swine and other animals. Agar test plates are provided including an underlying adherent coating of solubilized non-infectious swine pseudorabies antigen. Blood or blood serum samples from affected animals are placed in wells punched in the agar layer and allowed to incubate overnight. The agar gel is then removed. The resulting antigen layer with bound antibodies from the samples is washed and reacted with enzyme conjugated anti-swine immunoglobulin. The reaction is visualized by overlaying the bound conjugate layer with agar containing a color producing enzyme substrate. The diameters of resulting colored zones are correlated with the titers obtained by the official virus neutralization test. Methods of preparing antigen and antigen coated test plates are disclosed along with testing kits for carrying out the test procedure in the field.