Abstract: The present invention relates to the metabolic engineering of a microbial host for the synthesis of value-added products from oil palm empty fruit brunches (OPEFBs). In one embodiment, the genetically engineered microorganism is Escherichia coli comprising a metabolic pathway consisting of 9 enzymes (11 genes) to utilize depolymerized lignin, namely vanillin, p-coumaric acid, p-hydroxybenzaldehyde, vanillic acid, p-hydroxybenzoic acid and ferulic acid, to produce ?-ketoadipic acid, which can be subsequently converted into commercially important derivatives such as adipic acid and levulinic acid. The enzymes are feruloyl-CoA synthetase (fcs), enoyl-CoA hydratase (ech), vanillin dehydrogenase (vdh), vanillate O-demethylase (vanA; vanA and vanB), p-hydroxy benzoate hydroxylase (pobA), protocatechuate 3,4-dioxygenase {pcaGH; pcaG and pcaH), 3-carboxy-cis, cis-muconate cycloisomerase (pcaB), 4-carboxymuconolactone decarboxylase (pcaC), and ?-ketoadipate enol-lactone hydrolase (pcaD).

Abstract: An apparatus and method for stabilizing an output of a laser is provided. An optical amplifier oscillates light having a first wavelength band. A filter filters the light having the first wavelength band to output light having a second wavelength band narrower than the first wavelength band. The light having the second wavelength band may correspond to an output of a laser. To stabilize the wavelength center of the output laser, at least a portion of the light having the second wavelength band may be transferred to a fiber Bragg Grating (FBG) through a coupler. By performing feedback of a difference between the signal output from the FBG and a laser modulation signal, an offset corresponding to a lead zirconate titanate (PZT) operating parameter of a Fabry-Perot filter may be controlled to automatically stabilize the wavelength center.

Abstract: An apparatus and method for stabilizing an output of a laser is provided. An optical amplifier oscillates light having a first wavelength band. A filter filters the light having the first wavelength band to output light having a second wavelength band narrower than the first wavelength band. The light having the second wavelength band may correspond to an output of a laser. To stabilize the wavelength center of the output laser, at least a portion of the light having the second wavelength band may be transferred to a fiber Bragg Grating (FBG) through a coupler. By performing feedback of a difference between the signal output from the FBG and a laser modulation signal, an offset corresponding to a lead zirconate titanate (PZT) operating parameter of a Fabry-Perot filter may be controlled to automatically stabilize the wavelength center.