Abstract: The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.

Abstract: The present invention relates to a Vibrio harveyi-specific binding Dscam of shrimps, a method for identification thereof and a use thereof. Primarily, Dscam cDNAs are prepared from V. harveyi-challenged shrimps. A database is established with various Dscam isoforms therein by means of numerous cloning and sequencing. After a phylogenetic analysis, a plurality of candidate Dscam isoforms is selected. Each recombinant protein of candidate Dscam isoforms is expressed through an expression system. Finally, the binding affinity between V. harveyi and each recombinant protein of candidate Dscam isoforms is measured to further selection of a V. harveyi-specific binding Dscam and the amino acid sequence thereof. Accordingly, the Dscam can be specifically binding to V. harveyi for eliminating or reducing V. harveyi.

Abstract: The present invention relates to a WSSV peptide, which comprises a peptide sequence as set forth in SEQ ID NO: 1 and can be used as target peptide for WSD immunodetection in crustaceans. In addition, the present invention also provides a WSSV nucleotide sequence, which comprises a nucleotide sequence encoding the peptide sequence as set forth in SEQ ID NO: 1, and can be used as a detection target for WSD in crustaceans. On the other hand, the present invention can be applied in a WSSV detection method using the peptide or nucleotide sequences as targets. The WSSV peptide of the present invention is highly expressed in the hosts. The sensitivities of the WSSV detection using the target peptide in the invention are therefore greatly increased in comparison to the traditional detection target.

Abstract: The present invention relates to a WSSV peptide, which comprises a peptide sequence as set forth in SEQ ID NO: 1 and can be used as target peptide for WSD immunodetection in crustaceans. In addition, the present invention also provides a WSSV nucleotide sequence, which comprises a nucleotide sequence encoding the peptide sequence as set forth in SEQ ID NO: 1, and can be used as a detection target for WSD in crustaceans. On the other hand, the present invention can be applied in a WSSV detection method using the peptide or nucleotide sequences as targets. The WSSV peptide of the present invention is highly expressed in the hosts. The sensitivities of the WSSV detection using the target peptide in the invention are therefore greatly increased in comparison to the traditional detection target.