Patents by Inventor Hanna LEINONEN

Hanna LEINONEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Large-scale PEI-mediated plasmid transfection
    Patent number: 11781102
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: October 10, 2023
    Assignee: Trizell Ltd.
    Inventors: Hanna P. Lesch, Joonas Malinen, Eevi Lipponen, Anniina Valkama, Hanna Leinonen
  • Large-Scale PEI-Mediated Plasmid Transfection
    Publication number: 20200165557
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Application
    Filed: April 24, 2017
    Publication date: May 28, 2020
    Applicant: Trizell Ltd.
    Inventors: Hanna P. LESCH, Joonas MALINEN, Eevi LIPPONEN, Anniina VALKAMA, Hanna LEINONEN