Patents by Inventor Hanna P. Lesch

Hanna P. Lesch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Large-scale PEI-mediated plasmid transfection
    Patent number: 11781102
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: October 10, 2023
    Assignee: Trizell Ltd.
    Inventors: Hanna P. Lesch, Joonas Malinen, Eevi Lipponen, Anniina Valkama, Hanna Leinonen
  • FIXED-BED BIOREACTOR WITH CONSTANT-FLOW PUMP/ TUBING SYSTEM
    Publication number: 20220364036
    Abstract: We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.
    Type: Application
    Filed: December 22, 2021
    Publication date: November 17, 2022
    Inventors: Piia Kristiina Valonen, Hanna P. Lesch, Eva Kristiina Rasanen, Tarja Hannele Tuunanen, Minna Kristiina Karhinen, Seppo Ylä-Herttuala
  • VIRAL VECTOR ASSAY AND VECTOR
    Publication number: 20220081696
    Abstract: A process for assaying viral vector manufactured by large-scale viral vector manufacturing processes to assure the resulting vector has acceptable purity and potency.
    Type: Application
    Filed: July 28, 2021
    Publication date: March 17, 2022
    Inventors: Nigel Parker, Hanna P. Lesch, Jenni Mykkanen, Sara Paulo, Minna Hassinen, Robert Shaw
  • Fixed-bed bioreactor with constant-flow pump / tubing system
    Patent number: 11261416
    Abstract: We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.
    Type: Grant
    Filed: April 3, 2017
    Date of Patent: March 1, 2022
    Assignee: Trizell Ltd.
    Inventors: Piia Kristiina Valonen, Hanna P. Lesch, Eva Kristiina Rasanen, Tarja Hannele Tuunanen, Minna Kristiina Karhinen, Seppo Yla-Herttuala
  • Viral vector assay and vector
    Patent number: 11104915
    Abstract: A process for assaying viral vector manufactured by large-scale viral vector manufacturing processes to assure the resulting vector has acceptable purity and potency. The process entails three different types of assays, each one of which is optionally useful on a stand-alone basis, and which together provide the first system able to assure the quality of viral vector produced by large-scale vector manufacturing processes.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: August 31, 2021
    Assignee: Trizell Ltd.
    Inventors: Nigel Parker, Hanna P. Lesch, Jenni Mykkanen, Sara Paulo, Minna Hassinen, Robert Shaw
  • Nucleic acid application primers
    Patent number: 10829785
    Abstract: Novel forward primer, reverse primer and poly-linker suitable for replication of nucleic acids in e.g., 293 cells.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: November 10, 2020
    Assignee: Trizell Limited
    Inventors: Hanna P. Lesch, Kari J. Airenne, Seppo Yla-Herttuala
  • Large-Scale PEI-Mediated Plasmid Transfection
    Publication number: 20200165557
    Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
    Type: Application
    Filed: April 24, 2017
    Publication date: May 28, 2020
    Applicant: Trizell Ltd.
    Inventors: Hanna P. LESCH, Joonas MALINEN, Eevi LIPPONEN, Anniina VALKAMA, Hanna LEINONEN
  • Nucleic Acid Application Primers
    Publication number: 20200071725
    Abstract: Novel forward primer, reverse primer and poly-linker suitable for replication of nucleic acids in e.g., 293 cells.
    Type: Application
    Filed: November 13, 2019
    Publication date: March 5, 2020
    Applicant: Trizell Ltd.
    Inventors: Hanna P. Lesch, Kari J. Airenne, Seppo Yla-Herttuala
  • Viral Vector Assay and Vector
    Publication number: 20190338307
    Abstract: A process for assaying viral vector manufactured by large-scale viral vector manufacturing processes to assure the resulting vector has acceptable purity and potency. The process entails three different types of assays, each one of which is optionally useful on a stand-alone basis, and which together provide the first system able to assure the quality of viral vector produced by large-scale vector manufacturing processes.
    Type: Application
    Filed: September 9, 2016
    Publication date: November 7, 2019
    Applicant: Trizell Ltd.
    Inventors: Nigel PARKER, Hanna P. LESCH, Jenni MYKKANEN, Sara PAULO, Minna HASSINEN, Robert SHAW
  • Seeding An Adherent Cell Bioreactor With Non-Adherent Cells Increases Seeding Density Limit And Reduces Required Expansion Time
    Publication number: 20190276846
    Abstract: We have found a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells.
    Type: Application
    Filed: March 12, 2019
    Publication date: September 12, 2019
    Applicant: Trizell Ltd.
    Inventors: Eevi LIPPONEN, Hanna P. LESCH, Minna KARHINEN, Robert SHAW, Seppo YLA-HERTTUALA, Joonas MALINEN, Nigel PARKER
  • Fixed-Bed Bioreactor With Constant-Flow Pump / Tubing System
    Publication number: 20190031998
    Abstract: We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.
    Type: Application
    Filed: April 3, 2017
    Publication date: January 31, 2019
    Applicant: Trizell Ltd.
    Inventors: Piia Kristiina VALONEN, Hanna P. LESCH, Eva Kristiina RASANEN, Tarja Hannele TUUNANEN, Milla Jonna Karoliina KARNAATTU, Achim MULLER, Minna Kristiina KARHINEN
  • Seeding An Adherent Cell Bioreactor With Non-Adherent Cells Increases Seeding Density Limit And Reduces Required Expansion Time
    Publication number: 20170051309
    Abstract: We have found a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells
    Type: Application
    Filed: August 26, 2015
    Publication date: February 23, 2017
    Applicant: Trizell Ltd.
    Inventors: Hanna P. LESCH, Nigel PARKER, Minna KARHINEN, Robert SHAW, Seppo YLA-HERTTUALA, Jonas MALINEN, Eevi LIPPONEN
  • Production of Lentiviral Vectors
    Publication number: 20170016028
    Abstract: A high-volume gene therapy vector manufacturing process which produces a recombinant gene therapy vector which is able to transform host cells even when they are not dividing.
    Type: Application
    Filed: July 27, 2016
    Publication date: January 19, 2017
    Applicant: FinVector Vision Therapies Ltd.
    Inventors: Seppo YLA-HERTTUALA, Kari J. AIRENNE, Hanna P. LESCH
  • Production of Vectors for Non-Dividing Host Cells
    Publication number: 20160097060
    Abstract: A high-volume gene therapy vector manufacturing process, entailing using a recombinant baculovirus to transform a producer cell, which producer cell in turn produces a recombinant gene therapy vector which is able to transform host cells even when they are not dividing.
    Type: Application
    Filed: September 23, 2015
    Publication date: April 7, 2016
    Applicant: FinVector Vision Therapies Ltd.
    Inventors: Hanna P. LESCH, Kari J. Airenne, Seppo Yla-Herttuala
  • Production of Lentiviral Vectors
    Publication number: 20100003746
    Abstract: The present invention is a method of generating a lentivirus vector, comprising cloning each of a leotivimus transfer construct, gag, pol, an envelope protein and rev respectively into the same or different baculoviruses, and transducing a producer cell with the or each baculovirus.
    Type: Application
    Filed: February 11, 2008
    Publication date: January 7, 2010
    Inventors: Hanna P. Lesch, Kari J. Airenne, Seppo Yla-Herttuala