Abstract: This disclosure provides compositions that include a polynucleotide including or encoding a Cas12a tracrRNA, and methods for their use in sequence-specific genome editing, especially of eukaryotic genomic sequences. In particular, this disclosure provides Cas12a tracrRNA-containing compositions and methods for their use in Cas12a-mediated editing of a target sequence, wherein the editing efficiency is increased in comparison to controls lacking the Cas12a tracrRNA.

Abstract: A medical fluid removal apparatus and method uses negative pressure intraoperatively for the induction of hemostasis. The medical fluid removal apparatus includes a tissue interface membrane, a suction compliant body coupled to the tissue interface membrane, and an adapter configured for attachment to a negative pressure source. The suction compliant body is coupled between the tissue interface membrane and the adapter, and the tissue interface membrane is more flexible than the suction compliant body. The method of removing fluid during a medical procedure can include the steps of placing a tissue interface membrane of a medical fluid removal apparatus against a tissue of a patient, and suctioning the fluid beneath the tissue interface membrane or at least partially around the tissue interface membrane and through a suction compliant body of the medical fluid removal apparatus.

Abstract: Systems, methods, and compositions for providing improvements in plant gene-editing efficiency are provided.

Abstract: Compounds and related reagents, systems, methods, and compositions for increasing the frequency of homology directed repair (HDR) of target genomic sites with genome editing molecules are provided.

Abstract: Systems, methods, and compositions for providing improvements in plant gene-editing efficiency are provided.

Abstract: Systems, methods, and compositions that provide for increased frequencies of plant gene editing in comparison to controls are provided herein. Also disclosed are compositions that provide for editing target genes in plant cells. Systems for modification of a plant genome and/or target plant gene are provided. Methods for modifying a plant cell genome or a target plant gene in a plant cell genome are also provided. Compositions, plant cell cultures, and/or reaction mixtures comprising plant cells, certain agents, and gene editing molecules are also provided herein.

Abstract: This disclosure provides compositions that include a polynucleotide including or encoding a Cas12a tracrRNA, and methods for their use in sequence-specific genome editing, especially of eukaryotic genomic sequences. In particular, this disclosure provides Cas12a tracrRNA-containing compositions and methods for their use in Cas12a-mediated editing of a target sequence, wherein the editing efficiency is increased in comparison to controls lacking the Cas12a tracrRNA.

Abstract: This disclosure provides compositions that include a polynucleotide including or encoding a Cas12a tracrRNA, and methods for their use in sequence-specific genome editing, especially of eukaryotic genomic sequences. In particular, this disclosure provides Cas12a tracrRNA-containing compositions and methods for their use in Cas12a-mediated editing of a target sequence, wherein the editing efficiency is increased in comparison to controls lacking the Cas12a tracrRNA.

Abstract: This disclosure provides compositions that include a polynucleotide including or encoding a Cas12a tracrRNA, and methods for their use in sequence-specific genome editing, especially of eukaryotic genomic sequences. In particular, this disclosure provides Cas12a tracrRNA-containing compositions and methods for their use in Cas12a-mediated editing of a target sequence, wherein the editing efficiency is increased in comparison to controls lacking the Cas12a tracrRNA.

Abstract: Compounds and related reagents, systems, methods, and compositions for increasing the frequency of homology directed repair (HDR) of target genomic sites with genome editing molecules are provided.

Abstract: Systems, methods, and compositions that provide for increased frequencies of plant gene editing in comparison to controls are provided herein. Also disclosed are compositions that provide for editing target genes in plant cells. Systems for modification of a plant genome and/or target plant gene are provided. Methods for modifying a plant cell genome or a target plant gene in a plant cell genome are also provided. Compositions, plant cell cultures, and/or reaction mixtures comprising plant cells, certain agents, and gene editing molecules are also provided herein.