Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.

Abstract: The invention relates to a kit to be used for the identification of a secretion cassette, which in eukaryotic cells gives rise to a higher level of synthesis/secretion of a protein of interest, the coding sequence of which being flanked by the first and the second set of genetic elements, respectively, compared to the other secretion cassettes present in the kit containing the coding sequence at the corresponding position, as well as to a method wherein the kit is used and the use of the kit, whereby the best secretion cassette will be obtained.

Abstract: The invention relates to a method to generate rational libraries comprising genetic elements which are involved in transcriptional and/or translational regulation of a gene and devised to increase the production yield of the encoded protein as well as to the rational library and to the application of said rational library.

Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.

Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.

Abstract: The invention relates to a kit to be used for the identification of a secretion cassette, which in eukaryotic cells gives rise to a higher level of synthesis/secretion of a protein of interest, the coding sequence of which being flanked by the first and the second set of genetic elements, respectively, compared to the other secretion cassettes present in the kit containing the coding sequence at the corresponding position, as well as to a method wherein the kit is used and the use of said kit, whereby the best secretion cassette will be obtained.

Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.