Patents by Inventor Hans-Georg Hennemann
Hans-Georg Hennemann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10745721Abstract: The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2??(I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group, R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons, in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution, wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.Type: GrantFiled: November 12, 2013Date of Patent: August 18, 2020Assignee: Evonik Operations GmbHInventors: Steffen Schaffer, Heiko Andrea, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger
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Publication number: 20180155749Abstract: There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1) (E1) capable of hydrolysing at least one glyceride to at least one fatty acid wherein the glyceride is a triglyceride; and a second and a third genetic mutation that increases the expression relative to a wild type cell of: (i) an amino acid-N-acyl-transferase (EC 2.3.1) (E2), and (ii) an acyl-CoA synthetase (EC 6.2.1.3) (E3) respectively that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.Type: ApplicationFiled: April 28, 2016Publication date: June 7, 2018Inventors: Katrin GRAMMANN, Hans-Georg HENNEMANN, Nicole DECKER, Nicole KIRCHNER, Liv REINECKE
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Patent number: 9919303Abstract: The present invention relates to a method for removing an organic compound from an aqueous solution, comprising the steps of providing the aqueous solution which contains the organic compound, and a hydrophobic organic solution, where the latter comprises a liquid hydrophobic cation exchanger, contacting the aqueous solution and the hydrophobic organic solution, and separating off the hydrophobic organic solution from the aqueous solution, wherein the liquid hydrophobic cation exchanger is a saturated alkanoic acid having at least one alkyl substituent, where the organic compound is an organic compound having at least one positive charge and a neutral or positive total charge.Type: GrantFiled: July 24, 2013Date of Patent: March 20, 2018Assignee: Evonik Degussa GmbHInventors: Frank Erhardt, Lukas Falke, Ralf Kelle, Harald Haeger, Thomas Haas, Hans-Georg Hennemann, Oliver Thum, Martin Roos, Markus Poetter
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Patent number: 9765370Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.Type: GrantFiled: March 25, 2013Date of Patent: September 19, 2017Assignee: Evonik Degussa GmbHInventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
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Patent number: 9725746Abstract: The invention relates to a whole-cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an ?-dioxygenase and contacting the product with a transaminase.Type: GrantFiled: December 18, 2013Date of Patent: August 8, 2017Assignee: Evonik Degussa GmbHInventors: Steffen Schaffer, Jasmin Corthals, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Michael Volland, Martin Roos
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Patent number: 9719117Abstract: The invention provides a whole cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a ?-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.Type: GrantFiled: December 18, 2013Date of Patent: August 1, 2017Assignee: Evonik DegussaInventors: Steffen Schaffer, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
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Publication number: 20170130248Abstract: The present invention relates to a cell for producing acyl glycinates wherein the cell is genetically modified to comprise at least a first genetic mutation that increases the expression relative to the wild type cell of an amino acid-N-acyl-transferase, at least a second genetic mutation that increases the expression relative to the wild type cell of an acyl-CoA synthetase, and at least a third genetic mutation that decreases the expression relative to the wild type cell of at least one enzyme selected from the group consisting of an enzyme of the glycine cleavage system, glycine hydroxymethyltransferase (GlyA) and threonine aldolase (LtaE).Type: ApplicationFiled: April 28, 2015Publication date: May 11, 2017Applicant: Evonik Degussa GmbHInventors: LIV REINECKE, STEFFEN SCHAFFER, KATRIN GRAMMANN, MAIK OLFERT, NICOLE DECKER, NILS ARTO, HANS-GEORG HENNEMANN
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Patent number: 9611489Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C —(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.Type: GrantFiled: March 12, 2013Date of Patent: April 4, 2017Assignee: EVONIK DEGUSSA GmbHInventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
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Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium
Patent number: 9249435Abstract: A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of ?-oxidation of fatty acids.Type: GrantFiled: December 20, 2012Date of Patent: February 2, 2016Assignee: EVONIK DEGUSSA GmbHInventors: Jasmin Gielen, Thomas Haas, Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Katharina Berse, Marion Schiebelhut, Sabine Hafkemeyer, Markus Poetter, Frank Erhardt -
Patent number: 9200043Abstract: The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.Type: GrantFiled: March 15, 2011Date of Patent: December 1, 2015Assignee: Evonik Degussa GmbHInventors: Markus Pötter, Andreas Schmid, Bruno Bühler, Hans-Georg Hennemann, Mattijs Kamiel Julsing, Steffen Schaffer, Thomas Haas, Manfred Schrewe, Sjef Cornelissen, Martin Roos, Harald Häger
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Publication number: 20150307906Abstract: The invention relates to a whole-cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an ?-dioxygenase and contacting the product with a transaminase.Type: ApplicationFiled: December 18, 2013Publication date: October 29, 2015Applicant: Evonik Degussa GmbHInventors: Steffen SCHAFFER, Jasmin GIELEN, Mirja WESSEL, Hans-Georg HENNEMANN, Harald HÄGER, Michael VOLLAND, Martin ROOS
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Patent number: 9150890Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.Type: GrantFiled: January 30, 2015Date of Patent: October 6, 2015Assignee: EVONIK DEGUSSA GmbHInventors: Markus Poetter, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
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Publication number: 20150209775Abstract: The present invention relates to a method for removing an organic compound from an aqueous solution, comprising the steps of providing the aqueous solution which contains the organic compound, and a hydrophobic organic solution, where the latter comprises a liquid hydrophobic cation exchanger, contacting the aqueous solution and the hydrophobic organic solution, and separating off the hydrophobic organic solution from the aqueous solution, wherein the liquid hydrophobic cation exchanger is a saturated alkanoic acid having at least one alkyl substituent, where the organic compound is an organic compound having at least one positive charge and a neutral or positive total charge.Type: ApplicationFiled: July 24, 2013Publication date: July 30, 2015Applicant: Evonik Industries AGInventors: Frank Erhardt, Lukas Falke, Ralf Kelle, Harald Haeger, Thomas Haas, Hans-Georg Hennemann, Oliver Thum, Martin Roos, Markus Poetter
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Publication number: 20150140617Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.Type: ApplicationFiled: January 30, 2015Publication date: May 21, 2015Applicant: EVONIK DEGUSSA GMBHInventors: Markus POETTER, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
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Publication number: 20150111253Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.Type: ApplicationFiled: March 12, 2013Publication date: April 23, 2015Applicant: EVONIK INDUSTRIES AGInventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
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Publication number: 20150111254Abstract: The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell.Type: ApplicationFiled: March 25, 2013Publication date: April 23, 2015Applicant: EVONIK DEGUSSA GMBHInventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
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Patent number: 8999684Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.Type: GrantFiled: February 12, 2013Date of Patent: April 7, 2015Assignee: Evonik Degussa GmbHInventors: Markus Poetter, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
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Publication number: 20140256904Abstract: The subject of the invention is a biotechnological process for the production of ?-functionalized carboxylic acids and of ?-functionalized carboxylate esters from simple carbon sources.Type: ApplicationFiled: August 15, 2012Publication date: September 11, 2014Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Nicole Decker, Jasmin Gielen, Harald Haeger, Thomas Haas, Markus Poetter, Hans-Georg Hennemann, Mirja Wessel, Michael Volland
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Publication number: 20140178948Abstract: The invention provides a whole cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ?-hydroxy fatty acid, ?-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a ?-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.Type: ApplicationFiled: December 18, 2013Publication date: June 26, 2014Applicant: Evonik Industries AGInventors: Steffen SCHAFFER, Jasmin Gielen, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Thomas Haas, Wilfried Bluemke
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Publication number: 20140141478Abstract: The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2 ??(I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group,R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons,in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution,wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.Type: ApplicationFiled: November 12, 2013Publication date: May 22, 2014Applicant: Evonik Industries AGInventors: Steffen SCHAFFER, Heiko Andrea, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger