Patents by Inventor Hans Mollering
Hans Mollering has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5432070Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has1.) the nucleic acid sequence shown in FIG. (1),2.) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.Type: GrantFiled: June 10, 1994Date of Patent: July 11, 1995Assignee: Boehringer Mannheim GmbHInventors: Gunther Schumacher, Helmut Burtscher, Hans Mollering
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Patent number: 5213969Abstract: The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has(1) the nucleic acid sequence shown in FIG. (1),(2) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.Type: GrantFiled: July 8, 1991Date of Patent: May 25, 1993Assignee: Boehringer Mannheim, GmbHInventors: Gunther Schumacher, Helmut Burtscher, Hans Mollering
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Patent number: 4845029Abstract: The present invention provides a method of use for a hydrogen peroxide-forming oxidase, wherein the enzyme is obtained from Streptomycetaceae and at 25.degree. C., in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity. This enzyme is useful in the determination of sarcosine, creatine and creatinine.Type: GrantFiled: February 9, 1988Date of Patent: July 4, 1989Assignee: Boehringer Mannheim GmbHInventors: Ulrich Mayr, Hans Mollering, Joachim Siedel, Hans Seidel
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Patent number: 4816393Abstract: The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.Type: GrantFiled: February 25, 1985Date of Patent: March 28, 1989Assignee: Boehringer Mannheim GmbHInventors: Joachim Siedel, Rolf Deeg, Albert Roder, Joachim Ziegenhorn, Hans Mollering, Helmgard Gauhl
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Patent number: 4743549Abstract: The present invention provides a hydrogen peroxide-forming sarcosine oxidase, wherein it is obtainable from Streptomycetaceae and at 25.degree. C. in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity.Type: GrantFiled: May 28, 1986Date of Patent: May 10, 1988Assignee: Boehringer Mannheim GmbHInventors: Ulrich Mayr, Hans Mollering, Joachim Siedel, Hans Seidel
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Patent number: 4666832Abstract: The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions.The present invention also provides a process for preparing this new enzyme and a reagent containing it.Type: GrantFiled: February 27, 1984Date of Patent: May 19, 1987Assignee: Boehringer Mannheim GmbHInventors: Erich Elstner, Karl-Heinz Schleifer, Friedrich Gotz, Barbara Sedewitz, Albert Roder, Hans Mollering, Hans Seidel
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Patent number: 4416983Abstract: The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.Type: GrantFiled: December 7, 1981Date of Patent: November 22, 1983Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Joachim Siedel, Hans Mollering, Hans Seidel, Helmgard Gauhl
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Patent number: 4409328Abstract: For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.Type: GrantFiled: September 16, 1981Date of Patent: October 11, 1983Assignee: Boehringer Mannheim GmbHInventors: Joachim Ziegenhorn, Knut Bartl, Albert Roder, Gunter Lang, Hans Mollering, Ulrich Nagele
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Patent number: 4273870Abstract: Method and composition for the determination of glycerol using glycerin dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt and, in addition, at least one microgram of zinc, in the form of a zinc salt, per 50 U of glycerol dehydrogenase.Type: GrantFiled: July 5, 1979Date of Patent: June 16, 1981Assignee: Boehringer Mannheim GmbHInventors: Hans Mollering, Siegfried Looser
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Patent number: 4188465Abstract: Stabilized urease compositions are provided comprising urease and, as a stabilizing agent, a mixture of glutathione, ethylenediamine-tetraacetic acid, and citrate, preferably in a weight ratio of 1:0.5-5:0.5-3:0.5-3.Type: GrantFiled: March 25, 1977Date of Patent: February 12, 1980Assignee: Boehringer Mannheim GmbHInventors: Walter Schneider, Albert Roder, Hans Mollering, Ingeborg Gutmann
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Patent number: 4186052Abstract: A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.Type: GrantFiled: June 29, 1977Date of Patent: January 29, 1980Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Hans Mollering, Wolfgang Gruber, Klaus Beaucamp, Hans Seidel, Peter Stahl, Detlef von Hoerschelmann