Abstract: Methods and systems are provided for genotyping a gene sequence. The method comprises obtaining first genotyping call data representing a query gene sequence. A numerical score is assigned to each of a plurality of allele calls of second genotyping call data by matching the first genotyping call data with the second genotyping call data. the second genotyping call data representing a plurality of candidate gene sequences. A match score is determined for each of the plurality of candidate gene sequences based on the numerical score assigned to each of the plurality of allele calls of the second genotyping call data, and a genotyping call is made for the query gene sequence based on a highest match score from among the match score determined for each of the plurality of candidate gene sequences.

Abstract: The current disclosure provides for techniques and approaches for the generation of autologous mutant neoantigen-specific, TCR-engineered T cells used for adoptive transfer in treatment of cancer patients. Also provided are surrogate cancer cells, which is a personalized cell system that can be used for vaccination and TCR discovery in cancer patients.

Abstract: The disclosure provides Tn epitope-specific chimeric antigen receptors and scFvs, including soluble scFvs and multimeric scFvs, as well as methods of identifying cancer subjects and cancer subject sub-populations amenable to anti-Tn immunotherapy and methods of treating cancer.

Abstract: Disclosed are binding proteins, or fragments thereof, that specifically binds to a cancer-specific glycosylation variant of a protein and to a second epitope on the same protein, to a different protein presented on the same cell, or to a different protein presented on a different cell, such as an encoded polypeptide binding to both a cancer cell and an activated T cell. Also disclosed are polynucleotides encoding such binding proteins, including polynucleotides comprising codon-optimized coding regions and polynucleotides comprising coding regions that are not codon-optimized for expression in a particular host cell. Also disclosed are methods of making the encoded polypeptide and methods of using the polypeptide to treat, prevent or ameliorate the symptom of a disease such as cancer.

Abstract: Disclosed are binding proteins, or fragments thereof, that specifically binds to a cancer-specific glycosylation variant of a protein and to a second epitope on the same protein, to a different protein presented on the same cell, or to a different protein presented on a different cell, such as an encoded polypeptide binding to both a cancer cell and an activated T cell. Also disclosed are polynucleotides encoding such binding proteins, including polynucleotides comprising codon-optimized coding regions and polynucleotides comprising coding regions that are not codon-optimized for expression in a particular host cell. Also disclosed are methods of making the encoded polypeptide and methods of using the polypeptide to treat, prevent or ameliorate the symptom of a disease such as cancer.

Abstract: Disclosed are binding proteins, or fragments thereof, that specifically binds to a cancer-specific glycosylation variant of a protein and to a second epitope on the same protein, to a different protein presented on the same cell, or to a different protein presented on a different cell, such as an encoded polypeptide binding to both a cancer cell and an activated T cell. Also disclosed are polynucleotides encoding such binding proteins, including polynucleotides comprising codon-optimized coding regions and polynucleotides comprising coding regions that are not codon-optimized for expression in a particular host cell. Also disclosed are methods of making the encoded polypeptide and methods of using the polypeptide to treat, prevent or ameliorate the symptom of a disease such as cancer.

Abstract: The invention provides a methodology to use cancer vaccination to overcome tumor resistance to immunostimulatory antibodies. Our cancer vaccination approach relies on targeting mutant tumor-specific peptides that have high affinity for the major histocompatibility complex. Treatment with bacteria expressing high affinity tumor-specific peptides combined with anti-PD-L1 monoclonal antibody can eradicate long-established tumors resistant to anti-PD-L1 and anti-CTLA-4 alone. In addition, we discovered that adoptive transfer of T cells with this same vaccine-generated T cell receptor specificity can also eradicate long-established tumors. These results demonstrate that cancer vaccination approaches should target peptides with high peptide-MHC affinity to (i) overcome tumor resistance to immunostimulatory antibodies and (ii) identify T cell receptors that can be used successfully for adoptive T cell transfer.

Abstract: A convoluted boot for constant velocity joints and a constant velocity joint lubricant for use in such a convoluted boot. An additive which is added to the constant velocity joint lubricant and/or to the convoluted boot material is provided. The additive comprises amide waxes and also may include hydrocarbon oils. Use is made, in particular, of monoamides and/or diamides of carboxylic acids with 8 to 50 carbon atoms to form, by diffusion, a lubricating film on the surface of a convoluted boot. Furthermore, oleic acid amide and/or ethylenediamine distearate result in a particularly early formation of a lubricating film on the outer boot surface and thus to a long-term prevention of noise.

Abstract: The invention relates to an impregnating agent for a convoluted boot consisting of a thermplastic or elastomer material and used for covering the chamber of a joint, especially a rotating joint subjected to large articulation angles, which joint chamber is filled with a lubricant, said convoluted boot comprising a plurality of folds and two end collars, with the surfaces of the convoluted boot wall contacting one another when the joint is subjected to large articulation angles. It is the object of the invention to reduce the wear and eliminate squeaking symptoms of convoluted boots treated with the inventive impregnating agent. In accordance with the invention, the objective is achieved in that the impregnating agent contains a carrier fluid, an oil dissolved in the carrier fluid and having a molecular weight of >200, and a wetting agent which is soluble in oil and which reduces the surface tension of the oil.

Abstract: The invention relates to an impregnating agent for a convoluted boot consisting of a thermplastic or elastomer material and used for covering the chamber of a joint, especially a rotating joint subjected to large articulation angles, which joint chamber is filled with a lubricant, said convoluted boot comprising a plurality of folds and two end collars, with the surfaces of the convoluted boot wall contacting one another when the joint is subjected to large articulation angles. It is the object of the invention to reduce the wear and eliminate squeaking symptoms of convoluted boots treated with the inventive impregnating agent. In accordance with the invention, the objective is achieved in that the impregnating agent contains a carrier fluid, an oil dissolved in the carrier fluid and having a molecular weight of>200, and a wetting agent which is soluble in oil and which reduces the surface tension of the oil.

Abstract: A convoluted boot for constant velocity joints and a constant velocity joint lubricant for use in such a convoluted boot. An additive which is added to the constant velocity joint lubricant and/or to the convoluted boot material is provided. The additive comprises amide waxes and also may include hydrocarbon oils. Use is made, in particular, of monoamides and/or diamides of carboxylic acids with 8 to 50 carbon atoms to form, by diffusion, a lubricating film on the surface of a convoluted boot. Furthermore, oleic acid amide and/or ethylenediamine distearate result in a particularly early formation of a lubricating film on the outer boot surface and thus to a long-term prevention of noise.

Abstract: The invention relates to an impregnating agent for a convoluted boot consisting of a thermplastic or elastomer material and used for covering the chamber of a joint, especially a rotating joint subjected to large articulation angles, which joint chamber is filled with a lubricant, said convoluted boot comprising a plurality of folds and two end collars, with the surfaces of the convoluted boot wall contacting one another when the joint is subjected to large articulation angles. It is the object of the invention to reduce the wear and eliminate squeaking symptoms of convoluted boots treated with the inventive impregnating agent. In accordance with the invention, the objective is achieved in that the impregnating agent contains a carrier fluid, an oil dissolved in the carrier fluid and having a molecular weight of >200, and a wetting agent which is soluble in oil and which reduces the surface tension of the oil.

Abstract: A constant velocity universal ball joint (1) has an outer part (2), an inner part (6) received therein, and a cage (11) arranged between the outer part (2) and inner part (3) to guide balls (17) which serves to transmit torque between the outer part (2) and inner part (6). The balls (17) are guided between parallel window faces (15, 16) of the cage (11). To improve the service life values and the running-in conditions of the joint, balls (17) are used whose surface is ground and have a defined roughness. Thus, in the case of tolerance errors, the window faces (15, 16) are worked off to such an extent as to achieve identical load bearing conditions at the balls.

Abstract: Disclosed is a lubricating grease suitable for high temperature use, consisting essentially of 60 to 90% by weight of a base oil mixture comprising at least one mineral oil and at least one synthetic oil, 5 to 17% by weight of at least one urea compound as a thickener, wherein the at least one urea compound is a reaction product of at least one fatty amine and at least one isocyanate or at least one diisocyanate, 2 to 20% by weight of calcium complex grease, 1 to 4% by weight of molybdenum disulphide, 0.2 to 1% by weight of graphite powder, 0.2 to 1% by weight of polytetrafluoroethylene powder, 0.2 to 1% by weight of solid particles of at least one organo-molybdenum compound selected from a molybdenum dithiocarbamate and a molybdenum dithiophosphate, up to 2% by weight of a metal deactivator and up to 2% by weight of a corrosion inhibitor.

Abstract: In a safety system for a stepper-motor drive, a stepper motor for operating a throttle valve has poles with windings. Each pole winding is divided into part-windings, each of which is driven by a dedicated output stage. As a result, in the event of a failure of a part-winding or an output stage, it remains possible to control the stepper motor.

Abstract: A method and apparatus for performing a head-related model incorporating for transferring a jaw model between articulators of different types. The apparatus for the head-related incorporation of jaw models includes an articulator base plate, a ground plate connected to the articulator base plate and a cylinder having a cylindrical cavity connected vertically to an upper surface of the ground plate. A piston is inserted in the cylindrical cavity. A carrier plate is connected to the end of the piston opposite the articulator base plate. A split cast plate is positioned on the carrier plate and exactly repositionable via magnetic connection between the split cast plate and the carrier plate. A fixation element is connected to the carrier plate. An articulator mounting plate is supported by and connected to the split cast plate via a hardened viscous medium.

Abstract: With the present tank ventilation system formed of a fuel tank, an active carbon filter, a control unit, a lambda probe, a tank ventilation valve and a flow sensor it is possible to recognize defects at the connecting lines and the ventilation valve immediately. For the recognition of the defect, a control unit is provided which examines the signals arriving from the lambda probe and from the flow sensor along with the outgoing tank ventilation control signals for unreasonable events. When a defect is recognized, the error signal is stored. A ceramic PTC resistor is preferably employed as the flow sensor.

Abstract: A type characteristic of a measurement transducer is stored in a type memory of an evaluator. Deviations of the type characteristic from the individual characteristic of the measurement transducer are defined by individual values which accompany the measurement transducer in a coding memory. A computer of the evaluator identifies the corresponding measured value for every electrical measured signal from the type characteristic and from the individual values.

Abstract: A device for mounting for mounting jaw casts in an articulator, has a spatially adjustable cast plate, an articulator base plate, and wedges that can be moved longitudinally and transversely. The wedges can be rotated axially, and fixation elements can be moved like a sled in the inclination of the wedges. The fixation elements can be locked in a predetermined position by screwing in the inclination of the wedges, with the cast plate being pressed against the wedges and fixed.

Abstract: A method for producing and vulcanizing an inflatable hollow body from a double cloth having upper and lower plies with edges and pile yarns spacing the plies apart includes applying a respective gas-tight unvulcanized layer of rubber to each of the upper and lower piles of the double cloth. The edges of the upper and lower plies are joined with angle braces forming a hollow body. The hollow body is evacuated over a predetermined period of time and the hollow body is vulcanized in the evacuated state.