Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.

Abstract: The invention concerns a cryosurgical instrument with a probe for cooling limited zones of biological tissues, of which the thermally well-conducting tip has a hollow interior cross-section, into which a supply conduit connects with a constricted exit gap, through which a gas for cooling of the tip by means of the Joule-Thomson effect can be supplied by flow through the exit gap and can be led away therefrom through a coaxial discharge conduit open eventually to the atmosphere, the instrument also being provided with a device for control of the temperature of the probe tip.

Abstract: A heating coil close to the freezing tip of a cryosurgical probe operates through a highly heat conductive portion of the probe wall so as to cauterize previously frozen tissues. The liquid nitrogen supply is continued long enough to cool the cauterizing surface to body temperature after the heating winding is switched off, after which the probe is withdrawn. An insulating spacer member on the end of the tip prevents unintended freezing of the bladder wall when the instrument is used for an operation on the prostate gland. The use of general anesthesis in such an operation is avoided by the use of the new probe. A probe for cauterizing only, for use after freezing by a conventional cryosurgical probe has the same kind of heater, temperature sensor and insulating tip but is equipped for circulating cooling water rather than cryogenic flow in the cooling space.