Abstract: Embodiments of the present invention belong to the technical field of biomedicine, and specifically relate to a balasubramide derivative and use thereof in preparation of a medicine for treating acute lung injury. According to the embodiments of the present invention, it is proven through experiments that the balasubramide derivative can significantly reduce the expression of TNF-? in a macrophage cell line inflammation model, has no obvious toxicity at an effective dose, and has high safety. Moreover, the balasubramide derivative (+) 3C-20 can significantly relieve sepsis or acute lung injury induced by lung exposure to bacterial endotoxin, achieves the effect of relieving the acute lung injury of mice by inhibiting the expression of inflammatory factors in macrophages, and significantly improves the survival rate of model mice with acute lung injury.