Abstract: The invention is directed to a method to obtain the spatial location and sequence information of an m-RNA target sequence on a tissue sample comprising providing a solid surface, attaching anchor molecules, binding scaffolding molecules, incorporating adenine, guanine, cytosine and thymine, incorporating thymine to the anchor molecules, removing the scaffolding, providing a tissue sample, reverser transcrining to create c-DNA, removing the c-DNA and obtaining the sequence information of the c-DNA.

Abstract: The invention is directed to a method to obtain the spatial location and sequence information of a target sequence in a sample comprising at least one m-RNA strand comprising the steps a. providing a surface with a plurality of spacer units capable of binding at least one m-RNA strand and with at least one fiducial marker b. providing a sample comprising at least one m-RNA strand to the surface wherein at least one m-RNA strand of the sample binds to at least one spacer unit creating at least one single stranded oligomer c. taking a first image of the surface to obtain the spatial information of the sample relative to the fiducial marker d. removing sample form surface e. hybridizing at least one oligonucleotide comprising 50-1000 nucleic acids with its 5? and 3? ends to complementary parts of the single stranded oligomer thereby forming a padlock-shaped structure that is ligated to create a single strand circular template f.

Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target biological particle containing genomic material, and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid, and provided to a downstream workflow.

Abstract: Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.

Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target biological particle containing genomic material, and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid, and provided to a sequencing module. The sequencing module may sequence the genomic material and/or an identifying barcode attached to the bead.

Abstract: The present invention pertains to a method suitable for analyzing the temperature control of a device which is supposed to establish a defined temperature in a micro-environment, said method comprising a first Optical Temperature Verification step which comprises a) providing one or more thermochromatic liquid crystals in a micro-environment, wherein each thermochromatic liquid crystal has a specific event temperature, b) providing one or more temperature dependent luminophores in a micro-environment, c) varying the temperature in the micro-environments and irradiating the micro-environments with light, d) recording the luminescence of the one or more temperature dependent luminophores when the event temperature of the one or more thermochromatic liquid crystals is reached in the micro-environment and wherein said method preferably comprises a second Optical Temperature Verification step, which comprises the following a) providing one or more temperature dependent luminophores that were used in the First

Abstract: The present invention pertains to a method suitable for analysing the temperature control of a device which is supposed to establish a defined temperature in a micro-environment, said method comprising a first Optical Temperature Verification step which comprises a) providing one or more thermochromatic liquid crystals in a micro-environment, wherein each thermochromatic liquid crystal has a specific event temperature, b) providing one or more temperature dependent luminophores in a micro-environment, c) varying the temperature in the micro-environments and irradiating the micro-environments with light, d) recording the luminescence of the one or more temperature dependent luminophores when the event temperature of the one or more thermochromatic liquid crystals is reached in the micro-environment and wherein said method preferably comprises a second Optical Temperature Verification step, which comprises the following a) providing one or more temperature dependent luminophores that were used in the First