Patents by Inventor Hanxiao Jiang

Hanxiao Jiang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12098407
    Abstract: Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: September 24, 2024
    Assignee: Amyris Inc.
    Inventors: Penelope R. Chua, Joshua Adam Lerman, Thomas Jon Scherbart, Chandresh Thakker, Annie Ening Tsong, Hanxiao Jiang
  • Publication number: 20230066313
    Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.
    Type: Application
    Filed: January 22, 2021
    Publication date: March 2, 2023
    Inventors: Christopher J. PADDON, Hanxiao JIANG, Stephanie H. KUNG
  • Publication number: 20220315937
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Application
    Filed: June 15, 2022
    Publication date: October 6, 2022
    Inventor: Hanxiao JIANG
  • Publication number: 20220307059
    Abstract: Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.
    Type: Application
    Filed: June 5, 2020
    Publication date: September 29, 2022
    Inventors: Penelope R. CHUA, Joshua Adam LERMAN, Thomas Jon SCHERBART, Chandresh THAKKER, Annie Ening TSONG, Hanxiao JIANG
  • Patent number: 11390874
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: July 19, 2022
    Assignee: Amyris, Inc.
    Inventor: Hanxiao Jiang
  • Publication number: 20220106619
    Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
    Type: Application
    Filed: January 23, 2020
    Publication date: April 7, 2022
    Inventors: Gale A. WICHMANN, Sean LUND, Joshua LERMAN, Hanxiao JIANG, Yi XIONG
  • Publication number: 20210230234
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Application
    Filed: April 2, 2021
    Publication date: July 29, 2021
    Inventors: Penelope R. CHUA, Hanxiao JIANG, Adam Leon MEADOWS
  • Publication number: 20210155939
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Application
    Filed: January 27, 2021
    Publication date: May 27, 2021
    Inventors: Hanxiao JIANG, Adam MEADOWS
  • Patent number: 10988513
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: April 27, 2021
    Assignees: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
  • Patent number: 10927382
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: February 23, 2021
    Assignees: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Hanxiao Jiang, Adam Meadows
  • Publication number: 20200399326
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Application
    Filed: September 4, 2020
    Publication date: December 24, 2020
    Inventors: Penelope R. CHUA, Hanxiao JIANG, Adam Leon MEADOWS
  • Patent number: 10808015
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: October 20, 2020
    Assignee: AMYRIS, INC.
    Inventors: Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
  • Publication number: 20200208163
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Application
    Filed: September 12, 2018
    Publication date: July 2, 2020
    Inventor: Hanxiao JIANG
  • Publication number: 20180186841
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Application
    Filed: June 24, 2016
    Publication date: July 5, 2018
    Inventors: Penelope R. CHUA, Hanxiao JIANG, Adam Leon MEADOWS
  • Publication number: 20180171341
    Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
    Type: Application
    Filed: June 24, 2016
    Publication date: June 21, 2018
    Inventors: Penelope R. CHUA, Hanxiao JIANG, Adam Leon MEADOWS
  • Publication number: 20150299713
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Application
    Filed: August 7, 2013
    Publication date: October 22, 2015
    Applicants: Amyris, Inc., Total Marketing Services
    Inventors: Hanxiao Jiang, Adam Meadows