Abstract: The invention provides engineered mitochondria and a preparation method thereof, and relates to the technical field of mitochondria. The engineered mitochondria are formed by attaching exogenous cell membranes to outer membranes of exogenous mitochondria. The preparation method comprises the following steps: S1: extracting exogenous cell membranes from cells; S2: separating and extracting exogenous mitochondria from cells or tissue; and S3: mixing the separated and extracted exogenous mitochondria with the exogenous cell membranes in a specific ratio, thereby attaching the exogenous cell membranes to the outer membranes of the exogenous mitochondria to obtain the engineered mitochondria. The invention enables the production of engineered mitochondria with enhanced biological activity, exhibiting improved therapeutic effects on mitochondrial dysfunction-related disorders.

Abstract: The embodiments of the present disclosure provide a method for radiation therapy guidance. The method may include obtain first surface data of a target object to be treated by using an optical camera, wherein the first surface data may reflect the body surface condition of the target object before a treatment fraction; obtain a historical anatomical image of the target object; and determine whether an anatomical image of the target object needs to be reshot for the treatment fraction based on the historical anatomical image and the first surface data.

Abstract: The present disclosure is related to systems and methods for radiation. The method may include obtaining a plurality of reference images of a target of a subject and reference physiological motion information of the subject. The plurality of reference images and the reference physiological motion information may be acquired in a radiation period. The method may include establishing a correlation model based on the plurality of reference images and the reference physiological motion information. The method may include monitoring real-time motion information of the target based on the correlation model during a radiation operation performed during the radiation period.