Abstract: A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“?1st”) light using a grating; wherein the 1st light comprises spectral information and the ?1st light comprises spectral information; capturing the 1st light and the ?1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Abstract: A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“?1st”) light using a grating; wherein the 1st light comprises spectral information and the ?1st light comprises spectral information; capturing the 1st light and the ?1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Abstract: In an aspect, a method for additive manufacture of a three-dimensional object based on a computational model comprises steps of: grayscale photohardening a precursor material to form a portion of the object; and applying a hardened meniscus coating at a feature of the object; wherein the three-dimensional object is formed via at least the combination of the steps of gray scale photohardening and applying the meniscus coating. In some embodiments, the grayscale photohardening step is a grayscale photopolymerization step. In some embodiments, the applying a hardened meniscus coating step is a meniscus equilibrium post-curing step.

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and f) displaying the one or more non-diffraction limited images.

Abstract: The present disclosure provides systems and methods for imaging-guided monitoring and modeling of retinal vascular occlusive conditions. An example integrated optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) apparatus includes an OCT subsystem to acquire baseline OCT and OCT angiography (OCTA) volumes of a subject without dye before occlusion and subsequent OCT and OCTA volumes of the subject with dye after occlusion. The example apparatus includes an SLO subsystem including a laser controlled to adjust a laser to form a vascular occlusion at a location on a target vessel of the subject. The example apparatus includes a processor to process the OCT and OCTA volumes and feedback from the OCT subsystem and the SLO subsystem to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Systems and methods to measure blood flow using optical coherence tomography are disclosed. An example method includes: performing scanning of a target with beam(s) of incident low coherence radiation, wherein the low coherence radiation is selected from one or more regions of the electromagnetic spectrum based on the target selected; acquiring spectroscopic information from scanning signals generated by the interaction of the incident low coherence radiation and the target; generating image signal data for the target from the scanning signals; processing the image signal data by selecting electro-magnetic property(-ies) related to the modulation of the low coherence radiation by variation of relative permittivity and conductivity of the target; performing a multivariate comparison of the selected electro-magnetic properties related to the modulation of the low coherence radiation by variation of relative permittivity and conductivity of the target; and quantifying motion property(-ies) of the target.

Abstract: Systems and methods to generate spatially coherent electromagnetic radiation are disclosed. An example method includes receiving two or more incident wavelengths of electromagnetic radiation; applying the two or more incident wavelengths of electromagnetic radiation to an array of features; generating two or more spatially coherent optical resonating modes through the interaction of the one or more incident wavelengths of electromagnetic radiation and the array of features; and coupling the two or more spatially coherent optical resonating modes to two or more spatially coherent propagating wavelengths of electromagnetic radiation, wherein the spatially coherent propagating wavelengths of electromagnetic radiation are identical to the two or more incident wavelengths of electromagnetic radiation.

Abstract: The present disclosure provides systems and methods for imaging-guided monitoring and modeling of retinal vascular occlusive conditions. An example integrated optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) apparatus includes an OCT subsystem to acquire baseline OCT and OCT angiography (OCTA) volumes of a subject without dye before occlusion and subsequent OCT and OCTA volumes of the subject with dye after occlusion. The example apparatus includes an SLO subsystem including a laser controlled to adjust a laser to form a vascular occlusion at a location on a target vessel of the subject. The example apparatus includes a processor to process the OCT and OCTA volumes and feedback from the OCT subsystem and the SLO subsystem to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: The present disclosure provides systems and methods for objective focal length free measurements of fluid flow using OCT. In certain disclosed examples, fOCT data is acquired and optical information is extracted from fOCT scans to quantitatively determine a flow rate of fluid in the target. Determinations of flow rate can enable determination of a change in rate of an analyte over time. The current methods and systems of the disclosure can be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and, more generally, be used for the diagnosis, monitoring and treatment of disease.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: The devices, methods, and systems of the present disclosure provide for spectroscopic super-resolution microscopic imaging. In some examples, spectroscopic super-resolution microscopic imaging may be referred to or comprise spectroscopic photon localization microscopy (SPLM), a method which may employ the use of extrinsic labels or tags in a test sample suitable for imaging. In some examples spectroscopic super-resolution microscopic or spectroscopic photon localization microscopy (SPLM) may not employ extrinsic labels and be performed using the intrinsic contrast of the test sample or test sample material. Generally, spectroscopic super-resolution microscopic imaging may comprise resolving one or more non-diffraction limited images of an area of a test sample by acquiring both localization information of a subset of molecules using microscopic methods known in the art, and simultaneously or substantially simultaneously, acquiring spectral data about the same or corresponding molecules in the subset.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Systems, methods, and devices to fabricate one or more device components are disclosed. An example method includes fabricating one or more subject specific device components generated from receiving one or more images of one or more features of the first eye of the subject; designing a three dimensional virtual geometric model of the ophthalmic device using the one or more images; generating a plurality of virtual cross-sections of the three-dimensional virtual geometric model, wherein the cross-sections are defined by a set of physical parameters derived from the three-dimensional model; and fabricating the one or more subject specific features using the plurality of virtual cross-sections of the three dimensional model to direct an additive manufacturing method.

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and 0 displaying the one or more non-diffraction limited images.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Certain examples provide a transparent ultrasonic transducer including a transparent substrate and a transparent optical resonator positioned on the transparent substrate. The transparent optical resonator is to facilitate excitation of a biological sample and to receive acoustic emission from the biological sample triggered by the excitation, for example. Certain examples provide a ring resonator ultrasonic detector including a microscope cover slide. The microscope cover sheet includes a substrate and a ring optical resonator positioned on the substrate. The example ring optical resonator is to facilitate illumination of a biological sample and to receive acoustic emission from the biological sample in response to the illumination.

Abstract: Certain examples provide a structured illumination microscopy system. The example system includes a laser source to generate excitation illumination directed toward a target. The example system includes a modulator to modulate the excitation illumination temporally in a controllable spatial pattern to be constructed on the target object to provide sub-diffractional resolution in a lateral direction with respect to the target. The example system includes two synchronized laser scanning mirror units in confocal arrangement, the laser scanning units to be synchronized and controlled by a computing device, a first of the scanning mirror units to receive the modulated excitation illumination and project the modulated excitation illumination on the target object and a second of the scanning mirror units to receive emission fluorescence from the target and project the emission fluorescence.