Abstract: In an aspect, a method for additive manufacture of a three-dimensional object based on a computational model comprises steps of: grayscale photohardening a precursor material to form a portion of the object; and applying a hardened meniscus coating at a feature of the object; wherein the three-dimensional object is formed via at least the combination of the steps of gray scale photohardening and applying the meniscus coating. In some embodiments, the grayscale photohardening step is a grayscale photopolymerization step. In some embodiments, the applying a hardened meniscus coating step is a meniscus equilibrium post-curing step.

Abstract: An optical coherence tomography imaging system is disclosed, including: a light source to generate a radiation beam; a pair of photodetectors to acquire data of the radiation beam; a coupler to direct portions of the beam to a sample arm and a reference arm, the coupler to combine light from the sample arm and the reference arm, the combined light to be split into portions to be detected by the pair of photodetectors; and a processor to measure and compare noise profiles of the data and to generate an image using the data, and the noise profile comparison.

Abstract: Provided herein are systems and methods for additive manufacture of a customized ocular contact lens for a subject in a fast and convenient manner. In various aspects disclosed herein, methods disclosed herein provide for the imaging of the outer surface of a subject's cornea, designing a customized ocular contact lens based on the data from the imaging of the cornea, including determining anterior and posterior surfaces of the ocular contact lens, manufacturing the ocular contact lens using additive manufacturing, verifying the fit of the customized ocular contact lens on the subject's cornea, and verifying the optical power of the customized ocular contact lens. These features allow for the fast and convenient production of an ocular contact lens customized to a subject's cornea and manufactured to a specified optical power.

Abstract: The present disclosure provides a new visible light swept source that enables ultrafast visible light OCT to operate at a faster rate to reduce the motion artifacts and increase the field of view without sacrificing image quality. The new visible light swept source further provides for improved roll-off performance and mitigation of influence of RIN and wash-out effect for wide-field imaging. With a much-improved increase in imaging speed, increased signal-to-noise ratio (SNR), and increased imaging depth, visible light swept-source OCT (vis-ss-OCT) has the capability to perform more accurate functional and structural imaging.

Abstract: A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“?1st”) light using a grating; wherein the 1st light comprises spectral information and the ?1st light comprises spectral information; capturing the 1st light and the ?1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Abstract: A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“?1st”) light using a grating; wherein the 1st light comprises spectral information and the ?1st light comprises spectral information; capturing the 1st light and the ?1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Abstract: In an aspect, a method for additive manufacture of a three-dimensional object based on a computational model comprises steps of: grayscale photohardening a precursor material to form a portion of the object; and applying a hardened meniscus coating at a feature of the object; wherein the three-dimensional object is formed via at least the combination of the steps of gray scale photohardening and applying the meniscus coating. In some embodiments, the grayscale photohardening step is a grayscale photopolymerization step. In some embodiments, the applying a hardened meniscus coating step is a meniscus equilibrium post-curing step.

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and f) displaying the one or more non-diffraction limited images.

Abstract: The present disclosure provides systems and methods for imaging-guided monitoring and modeling of retinal vascular occlusive conditions. An example integrated optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) apparatus includes an OCT subsystem to acquire baseline OCT and OCT angiography (OCTA) volumes of a subject without dye before occlusion and subsequent OCT and OCTA volumes of the subject with dye after occlusion. The example apparatus includes an SLO subsystem including a laser controlled to adjust a laser to form a vascular occlusion at a location on a target vessel of the subject. The example apparatus includes a processor to process the OCT and OCTA volumes and feedback from the OCT subsystem and the SLO subsystem to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Systems and methods to measure blood flow using optical coherence tomography are disclosed. An example method includes: performing scanning of a target with beam(s) of incident low coherence radiation, wherein the low coherence radiation is selected from one or more regions of the electromagnetic spectrum based on the target selected; acquiring spectroscopic information from scanning signals generated by the interaction of the incident low coherence radiation and the target; generating image signal data for the target from the scanning signals; processing the image signal data by selecting electro-magnetic property(-ies) related to the modulation of the low coherence radiation by variation of relative permittivity and conductivity of the target; performing a multivariate comparison of the selected electro-magnetic properties related to the modulation of the low coherence radiation by variation of relative permittivity and conductivity of the target; and quantifying motion property(-ies) of the target.

Abstract: Systems and methods to generate spatially coherent electromagnetic radiation are disclosed. An example method includes receiving two or more incident wavelengths of electromagnetic radiation; applying the two or more incident wavelengths of electromagnetic radiation to an array of features; generating two or more spatially coherent optical resonating modes through the interaction of the one or more incident wavelengths of electromagnetic radiation and the array of features; and coupling the two or more spatially coherent optical resonating modes to two or more spatially coherent propagating wavelengths of electromagnetic radiation, wherein the spatially coherent propagating wavelengths of electromagnetic radiation are identical to the two or more incident wavelengths of electromagnetic radiation.

Abstract: The present disclosure provides systems and methods for imaging-guided monitoring and modeling of retinal vascular occlusive conditions. An example integrated optical coherence tomography (OCT) and scanning laser ophthalmoscope (SLO) apparatus includes an OCT subsystem to acquire baseline OCT and OCT angiography (OCTA) volumes of a subject without dye before occlusion and subsequent OCT and OCTA volumes of the subject with dye after occlusion. The example apparatus includes an SLO subsystem including a laser controlled to adjust a laser to form a vascular occlusion at a location on a target vessel of the subject. The example apparatus includes a processor to process the OCT and OCTA volumes and feedback from the OCT subsystem and the SLO subsystem to determine a change in three-dimensional vasculature from before the vascular occlusion to after the vascular occlusion.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: The present disclosure provides systems and methods for objective focal length free measurements of fluid flow using OCT. In certain disclosed examples, fOCT data is acquired and optical information is extracted from fOCT scans to quantitatively determine a flow rate of fluid in the target. Determinations of flow rate can enable determination of a change in rate of an analyte over time. The current methods and systems of the disclosure can be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and, more generally, be used for the diagnosis, monitoring and treatment of disease.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine both a flow rate of fluid in the target and a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: The devices, methods, and systems of the present disclosure provide for spectroscopic super-resolution microscopic imaging. In some examples, spectroscopic super-resolution microscopic imaging may be referred to or comprise spectroscopic photon localization microscopy (SPLM), a method which may employ the use of extrinsic labels or tags in a test sample suitable for imaging. In some examples spectroscopic super-resolution microscopic or spectroscopic photon localization microscopy (SPLM) may not employ extrinsic labels and be performed using the intrinsic contrast of the test sample or test sample material. Generally, spectroscopic super-resolution microscopic imaging may comprise resolving one or more non-diffraction limited images of an area of a test sample by acquiring both localization information of a subset of molecules using microscopic methods known in the art, and simultaneously or substantially simultaneously, acquiring spectral data about the same or corresponding molecules in the subset.

Abstract: The present disclosure provides systems and methods for the determining a rate of change of one or more analyte concentrations in a target using non invasive non contact imaging techniques such as OCT. Generally, OCT data is acquired and optical information is extracted from OCT scans to quantitatively determine a flow rate of fluid in the target; angiography is also performed using one or more fast scanning methods to determine a concentration of one or more analytes. Both calculations can provide a means to determine a change in rate of an analyte over time. Example methods and systems of the disclosure may be used in assessing metabolism of a tissue, where oxygen is the analyte detected, or other functional states, and be generally used for the diagnosis, monitoring and treatment of disease.

Abstract: Systems, methods, and devices to fabricate one or more device components are disclosed. An example method includes fabricating one or more subject specific device components generated from receiving one or more images of one or more features of the first eye of the subject; designing a three dimensional virtual geometric model of the ophthalmic device using the one or more images; generating a plurality of virtual cross-sections of the three-dimensional virtual geometric model, wherein the cross-sections are defined by a set of physical parameters derived from the three-dimensional model; and fabricating the one or more subject specific features using the plurality of virtual cross-sections of the three dimensional model to direct an additive manufacturing method.

Abstract: Certain examples disclose systems and methods for imaging a target. An example method includes: a) activating a subset of light-emitting molecules in a wide field area of a target using an excitation light; b) capturing one or more images of the light emitted from the subset of the molecules illuminated with the excitation light; c) localizing one or more activated light emitting molecules using one or more single molecule microscopic methods to obtain localization information; d) simultaneously capturing spectral information for the same localized activated light emitting molecules using one or more spectroscopic methods; e) resolving one or more non-diffraction limited images of the area of the target using a combination of the localization and spectral information for the localized activated light emitting molecules; and 0 displaying the one or more non-diffraction limited images.